The SpinPrep™ PCR Clean-Up Kit is designed for the rapid purification of DNA amplified in PCR. This procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low-salt buffer. This kit removes DNA polymerases, dNTPs, salts, and > 99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labeling. PCR products from 100 bp to > 12,000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60-90% under standard conditions.

• Rapid purification of PCR products for downstream procedures
• Time required is less than ten minutes
• PCR volume is about 100 µl per reaction