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SpinPrep™, PCR Clean-up Kit, MilliporeSigma
SpinPrep™, PCR Clean-up Kit, MilliporeSigma
Catalog # CA80500-372
Supplier:  MilliporeSigma
CAS Number:  
SpinPrep™, PCR Clean-up Kit, MilliporeSigma
Catalog # CA80500-372
Supplier:  MilliporeSigma
Supplier Number:  70976-3
CAS Number:  

Specifications

  • Method / Format:
    Column (silica-based)
  • Prep size:
    Mini
  • Refine target molecule:
    Amplified/modified DNA
  • Sample type:
    PCR and other enzymatic reactions
  • Target molecule:
    DNA
  • Description:
    SpinPrep™ PCR Clean-up Kit
  • Size:
    100 reactions
  • Binding capacity:
    Up to 6 µg
  • Description:
    SpinPrep™ PCR Clean-up Kit
  • Cat. no.:
    CA80500-372
  • Volume:
    100 µl
  • Supplier No.:
    70976-3

Specifications

About this item

The SpinPrep™ PCR Clean-Up Kit is designed for the rapid purification of DNA amplified in PCR. This procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low-salt buffer. This kit removes DNA polymerases, dNTPs, salts, and > 99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labeling. PCR products from 100 bp to > 12,000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60-90% under standard conditions.

  • Rapid purification of PCR products for downstream procedures
  • Time required is less than ten minutes
  • PCR volume is about 100 µl per reaction