SpinPrep™, PCR Clean-up Kit, MilliporeSigma
Catalog # CA80500-372
Supplier: MilliporeSigma
CAS Number:
Specifications
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Amplified/modified DNA
- Sample type:PCR and other enzymatic reactions
- Target molecule:DNA
- Description:SpinPrep™ PCR Clean-up Kit
- Size:100 reactions
- Binding capacity:Up to 6 µg
- Description:SpinPrep™ PCR Clean-up Kit
- Cat. no.:CA80500-372
- Volume:100 µl
- Supplier No.:70976-3
Specifications
About this item
The SpinPrep™ PCR Clean-Up Kit is designed for the rapid purification of DNA amplified in PCR. This procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low-salt buffer. This kit removes DNA polymerases, dNTPs, salts, and > 99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labeling. PCR products from 100 bp to > 12,000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60-90% under standard conditions.
- Rapid purification of PCR products for downstream procedures
- Time required is less than ten minutes
- PCR volume is about 100 µl per reaction