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- Assay Type:Competitive
- Target protein:cGMP
- Sensitivity:Assay Buffer : 0.42pmol/mL (non-acetylated) 0.043pmol/mL (acetylated) HCl: 0.604pmol/mL (non-acetylated) 0.059pmol/mL (acetylated) (range 0.8 - 500pmol/mL (non-acetylated); 0.08 - 50pmol/mL (acetylated))
- Cat. no.:89141-242
- Supplier No.:900-164
- No. of Tests:96 wells
Most sensitive (0.043pmol/m) and complete colorimetric kit for intra- and extracellular cGMP quantification.
For the quantitative determination of cGMP in cell lysates, culture supernatants, plasma, serum, saliva, tissue, and urine from any species.
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP diluted in buffer or intracellular cGMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.