The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E
- Elute in as little as 6 μL
- Eliminate buffer retention and salt carryover with optimized column design
- Save time with fast, user-friendly protocols
- No need to monitor the pH of your reaction
- Reduce your impact on the environment
- Use less plastic
- Responsibly-sourced, recyclable packaging
- Quickly purify DNA from agarose gels
It was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–80 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format.
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- Application:
- Environmentally Preferable:
: VWR International
: VWR International
: VWR International
: VWR International