Compared to other basal media, Ham’s F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Ham’s F-12 contains no proteins or growth factors, and uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH.

• Without hypoxanthine and L-glutamine

Ham’s F-12 Nutrient Mixture without Hypoxanthine is useful for serum free clonal growth of Chinese Hamster Ovary (CHO) cells, chick embryos and mouse L-cells. Ham’s F-12 Nutrient Mixture without Hypoxanthine can also be used to culture myeloma cells and hybridomas, as well as primary rat hepatocytes and prostate epithelial cells.

Recommendation
Add L-glutamine prior to use at a final concentration of 1.0 mM.
Supplement 10% FBS because F-12 contains no proteins or growth factors.

Sterile Filtered
Harmonization Code: 3821.00.0000
ECCN: EAR99