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202 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Pierce™ Controlled Protein-Protein Cross-Linking Kit, Thermo Scientific

Pierce™ Controlled Protein-Protein Cross-Linking Kit, Thermo Scientific

Supplier: Thermo Scientific

The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.They insert an unbreakable molecular bridge between two proteins with easy-to-control stepwise coupling.

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Pierce™ Dye Removal Columns, Thermo Scientific

Pierce™ Dye Removal Columns, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

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Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific

Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Thermo Scientific

Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

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Amine Reactive Biotinylation Kits, EZ-Link™, Thermo Scientific

Amine Reactive Biotinylation Kits, EZ-Link™, Thermo Scientific

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

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Pierce™ Pegylation Reagents, Methyl-PEG-Amine Compounds, Thermo Scientific

Pierce™ Pegylation Reagents, Methyl-PEG-Amine Compounds, Thermo Scientific

Supplier: Thermo Scientific

The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers

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Pierce™ Immunoprecipitation, Crosslink IP Kit, Thermo Scientific

Pierce™ Immunoprecipitation, Crosslink IP Kit, Thermo Scientific

Supplier: Thermo Scientific

The Pierce™ Crosslink Immunoprecipitation (IP) Kit enables highly effective and efficient antigen immunoprecipitations by covalently crosslinking antibodies onto Protein A/G resin.

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Template Switching RT Enzyme Mix, New England Biolabs

Template Switching RT Enzyme Mix, New England Biolabs

Supplier: New England Biolabs (NEB)

The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template.

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Pierce™  ABC Peroxidase Standard Staining Kit, Thermo Scientific

Pierce™ ABC Peroxidase Standard Staining Kit, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

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Pierce™ PEGylated SPDP Crosslinkers, Thermo Scientific

Pierce™ PEGylated SPDP Crosslinkers, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce™ PEGn-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a n-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

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SDC™ (Steroid/Drug/Compound) Immobilization Kit, G-Biosciences

SDC™ (Steroid/Drug/Compound) Immobilization Kit, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification

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Recombinant Streptavidin, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' recombinant streptavidin comes from streptomyces avidinii, produced in E.coli

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Pierce™ PEGylated BM (bismaleimido) Crosslinkers, Thermo Scientific

Pierce™ PEGylated BM (bismaleimido) Crosslinkers, Thermo Scientific

Supplier: Thermo Scientific

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions

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Pierce™ GlycoLink™ Coupling Catalyst, Thermo Scientific

Pierce™ GlycoLink™ Coupling Catalyst, Thermo Scientific

Supplier: Thermo Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

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SNAP-CELL® Block , New England Biolabs

SNAP-CELL® Block , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Cell® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag® in solution or in living cells

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SNAP-SURFACE® Block , New England Biolabs

SNAP-SURFACE® Block , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells

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IMPACT™ Kit for Protein Purification, New England Biolabs

IMPACT™ Kit for Protein Purification, New England Biolabs

Supplier: New England Biolabs (NEB)

The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.

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Streptavidin, New England Biolabs

Streptavidin, New England Biolabs

Supplier: New England Biolabs (NEB)

A 52.8 kDa non-glycosylated protein with a very high affinity for biotin

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Pierce™ Sulfhydryl Addition Kit, Thermo Scientific

Pierce™ Sulfhydryl Addition Kit, Thermo Scientific

Supplier: Thermo Scientific

Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.

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Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific

Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific

Supplier: Thermo Scientific

The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers

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CLIP-Biotin, New England Biolabs

CLIP-Biotin, New England Biolabs

Supplier: New England Biolabs (NEB)

CLIP-Biotin is a cell-permeable substrate (BC-Biotin) based on biotin with an amidocaproyl linker

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Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific

Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific

Supplier: Thermo Scientific

DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.

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Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers, Thermo Scientific

Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers, Thermo Scientific

Supplier: Thermo Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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