- Antibody type:Primary
- Antigen name:transcription factor A, mitochondrial
- Antigen symbol:Tfam
- Clonality:Polyclonal
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Rabbit
- ImmunoChemistry:Yes
- Reactivity:Rat,Mouse
- Western blot:Yes
- Size:100 ul
- Form:Liquid
- Gene ID:P40630
- Antigen synonyms:Tfam
- Storage buffer:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Molecular weight:27 kDa
- Storage temperature:For short periods of storage (days) store at 4 °C. For longer periods of storage, store Tfam antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
- Shipping temperature:4 °C
- Immunogen:Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse Tfam.
- Purification:Antibody is purified by peptide affinity chromatography method.
- Cat. no.:10106-584
- Supplier No.:28-598
TFAM a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this protein is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns Sayre syndrome was produced when expression of the gene was eliminated by targeted disruption in heart and muscle cells.
- Tfam antibody can be used for detection of Tfam by ELISA at 1:312500. Tfam antibody can be used for detection of Tfam by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is purified by peptide affinity chromatography method.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: Tfam
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Mouse, Rat