IBI's Maxi Fast-Ion Plasmid Kits are endotoxin free and use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures

• Sample Size: 100 ml high-copy plasmid/250 ml low-copy plasmid
• Operation: Gravity Flow Column
• Expectant Yield: 500 µg to 1mg of plasmid DNA
• Operation Time: 120 minutes or less

The Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than two hours. The purified plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.

In this process, the modified alkaline lysis method and Rnase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting.