IBI's rYeast Mini Total RNA Kits have been designed for purifying total RNA from yeast and a variety of other fungi

• Sample Size: up to 5×10⁷ fungus cells
• Expected Yield: up to 30 µg of RNA
• Elution Volume: 50-100 µl
• Operation Time: within 70 minutes

The rYeast Total RNA Mini Kit was designed for total RNA purification from yeast and a wide variety of other fungus species. Sorbitol Buffer is included with the kit to reduce sample preparation time and minimize hands on time. Detergents and chaotropic salt are used to lyse cells and inactivate RNase while RNA is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free water. The entire procedure can be completed within 70 minutes and the purified RNA is ready-for-use in RT-PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis.