You searched for: Cell Culture Supplements and Additives
The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.
ReagentPack® Chondrocyte Subculture Reagents
Supplier: LONZA PHARMA - BIOSCIENCE CA
Chondrocyte ReagentPack® Subculture Kits have been designed specifically for the passaging of primary chondrocytes.
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Halt™ Phosphatase Inhibitor Cocktail, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Halt Phosphatase Inhibitor Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction.
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A23187, Mixed Calcium-Magnesium Salt, Calbiochem
Supplier: MilliporeSigma
Useful in cell activation experiments when calcium dose-response data are not required.
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Trypsin/EDTA Solution
Supplier: LONZA PHARMA - BIOSCIENCE CA
Trypsin is a clear, colorless-yellow, highly active bovine enzyme preparation, used to remove cells from the culture vessel surface into suspension.It is generally used at a working concentration of 0.025%, prepared in a balanced salt solution, without calcium or magnesium at a pH of 7.6 - 7.8.
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TM-235 Defined Serum-Free Growth Medium, Protide Pharmaceuticals
Supplier: PROTIDE PHARMACEUTICALS
Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.
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Bovine Brain Extract (BBE)
Supplier: LONZA PHARMA - BIOSCIENCE CA
Bovine Brain Extract is useful in the culture of various endothelial cell types. The product is offered at a concentration of 9 mg/ml.
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Protease Inhibitor Cocktail Set III, EDTA-Free, MilliporeSigma
Supplier: MilliporeSigma
A cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases
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Protease Inhibitor Cocktail Set I, MilliporeSigma
Supplier: MilliporeSigma
A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases
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Protease Inhibitor Cocktail VI, Plant Cells, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Storage Temperature: -30°C to -10°C
Physical Form: Liquid
Appearance: Clear
UN No.:UN2922
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Ascorbic Acid
Supplier: LONZA PHARMA - BIOSCIENCE CA
Ascorbic Acid is an important component in various primary cell culture media kits. It is included in various BulletKits. Ascorbic Acid is also sold separately at a concentration of 70 mM.
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Halt™ Protease Inhibitor Cocktail (100X), Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Halt Protease Inhibitor Cocktail (100X) are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction from animal and plant tissues, cultured cells,
yeast or bacteria.
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SingleQuots® Neural Progenitor Supplements
Supplier: LONZA PHARMA - BIOSCIENCE CA
Supplements for Neural Progenitor medium.
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MCl-186, Calbiochem
Supplier: MilliporeSigma
A free radical scavenger and antioxidant that reduces post-ischemic brain injury
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Cellvation DMSO Free Cryopreservation Solution, Protide Pharmaceuticals
Supplier: PROTIDE PHARMACEUTICALS
A unique cryopreservation solution that does not contain DMSO or fetal bovine serum. Cellvation™ is convenient to use and provides optimal recovery. Cells can be exposed to Cellvation™ for longer periods of time before the freezing process is initiated. Cellvation™ is ready to use and can be used for cells cultured in serum-free medium or with serum containing medium.
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Pierce™ Universal Nuclease for Cell Lysis
Supplier: Thermo Scientific
The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.
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2D Electrophoresis Reagents, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' FOCUS™ Protein Alkylation Kit contains an ultra pure, proteomic grade iodoacetamide reagent for blocking thiols by alkylation in order to prevent the reoxidation of proteins
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TCH Defined Serum-Free Growth Medium, Protide Pharmaceuticals
Supplier: PROTIDE PHARMACEUTICALS
Serum replacement used primarily for human cells and the production of cell secreted proteins. TCH™ is also effective in culturing other mammalian cells without the use of animal proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, recombinant insulin), which are heat treated.
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NSF-1 Neural Survival Factor-1
Supplier: LONZA PHARMA - BIOSCIENCE CA
NSF-1 Neural Survival Factor-1.
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Gentamicin sulfate-Amphotericin (GA-1000)
Supplier: LONZA PHARMA - BIOSCIENCE CA
GA-1000 vial contains 30 mg/ml Gentamicin and 15 µg/ml Amphotericin. We recommend use at a 1:1000 ratio (i.e. 0.2 ml into a 200 ml bottle for a final concentration of 30 µg/ml Gentamicin and 15 µg/ml Amphotericin).
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Bovine Pituitary Extract (BPE)
Supplier: LONZA PHARMA - BIOSCIENCE CA
Bovine Pituitary Extract is useful in the culture of various epithelial cell types. The product is included in SingleQuots® Kits associated with various media systems for epithelial cell types. The product is available at a concentration of 13 mg/ml.
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Protease Inhibitor Cocktail III, Animal-Free, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Storage Temperature: -30°C to -10°C
Physical Form: Liquid
Appearance: Clear
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ProteaseArrest™ Protease Inhibitor Cocktails, G-Biosciences
Supplier: G-Biosciences
ProteaseArrest™ is a general protease inhibitor cocktail solution that is provided as a 100X concentrated, ready-to-use solution
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Calcium Chloride, 300 mM
Supplier: LONZA PHARMA - BIOSCIENCE CA
Calcium Chloride is available at a concentration of 300mM solution. This is included for use in select primary cell culture media kits.
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SingleQuots® BEGM® Bronchial Epithelial Kit
Supplier: LONZA PHARMA - BIOSCIENCE CA
BEGM® SingleQuot® Kits are components sold separately and does not include the basal medium. These are part of the BEGM® Bronchial Epithelial Cell Growth Medium BulletKit® (catalog # CC-3170) which consists of basal medium and supplements packaged together.
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ReagentPack® Subculture Reagents
Supplier: LONZA PHARMA - BIOSCIENCE CA
ReagentPack® Subculture Kits have been designed specifically for the passaging of primary cell types. Each kit contains 100 mL each of: Trypsin/EDTA, Trypsin Neutralizing Solution, HEPES Buffered Saline.
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N-tert-Butyl-[alpha]-phenylnitrone, Calbiochem®
Supplier: MilliporeSigma
Most commonly-used free radical trap
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Protease Inhibitor Cocktail II, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Storage Temperature: -30°C to -10°C
Physical Form: Lyophilized solid
Sensitivity: Hygroscopic
Appearance: White
UN No.:UN3261
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Proteasome Inhibitor Set, Calbiochem®
Supplier: MilliporeSigma
Set contains 1mg of Proteasome Inhibitor I (80053-874, -876), 200µg of Lactacystin (80052-806), and 1mg of MG-132 (80053-194, -196)
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SOC Outgrowth Medium, New England Biolabs
Supplier: New England Biolabs (NEB)
Add SOC to freshly transformed E.coli cells and incubate for up to 1 hour before plating on selective media.