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202 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit

ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST INC

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Biotin-16-dUTP, Biotium

Biotin-16-dUTP, Biotium

Supplier: Biotium

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.

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Annexin V, CF® 770 conjugate

Annexin V, CF® 770 conjugate

Supplier: Biotium

Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.

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Allophycocyanin (Crosslinked APC), Columbia Biosciences

Allophycocyanin (Crosslinked APC), Columbia Biosciences

Supplier: Columbia Biosciences

Allophycocyanin (APC) is a commonly used label in Time-Resolved Fluorescence Resonant Energy Transfer (TR-FRET) assays for High Throughput Screening and Flow Cytometry.

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Reactive Dye Conjugates, Biotium

Supplier: Biotium

Reactive dyes containing an amine functional group.

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HRP WGA Wheat Germ Agglutinin (WGA) Conjugates

HRP WGA Wheat Germ Agglutinin (WGA) Conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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CF®405S BCN

CF®405S BCN

Supplier: Biotium

CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.

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CF®680 Azide

CF®680 Azide

Supplier: Biotium

CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

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CF™ Dye Lectin Conjugates, Biotium

CF™ Dye Lectin Conjugates, Biotium

Supplier: Biotium

A lectin labeled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

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Phalloidin Conjugates, Biotium

Phalloidin Conjugates, Biotium

Supplier: Biotium

A highly selective actin filament stain for fixed and permeabilized cells. Labeled phalloidins have similar affinity for both large and small filaments.

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ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST INC

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Thiol-Reactive Fluorescent Dyes and Other Thiol-Modifying Reagents, Biotium

Thiol-Reactive Fluorescent Dyes and Other Thiol-Modifying Reagents, Biotium

Supplier: Biotium

MTS, TS, and maleimide, and other thiol-modifying reagents and reactive fluorescent dyes.

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Biotin-20-dUTP, Biotium

Biotin-20-dUTP, Biotium

Supplier: Biotium

Biotin-20-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.

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VivoBrite™ rapid antibody labeling kit for small animal in vivo imaging, CF™790 SE

VivoBrite™ rapid antibody labeling kit for small animal in vivo imaging, CF™790 SE

Supplier: Biotium

Near-infrared dyes offer highly sensitive and specific detection in complex biological systems, because cells and tissues have intrinsically low autofluorescence in the near-IR region

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EZ-Link™ Desthiobiotinylation and Pull-Down Kit and Reagents, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific EZ-Link™ Desthiobiotinylation and Pull-down Kit is a complete set of reagents to biotin-label purified proteins and use them to purify interaction complexes using streptavidin agarose resin with mild elution conditions.

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Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits, Biotium

Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits, Biotium

Supplier: Biotium

Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.

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Reactive CF® Dyes and Protein Labeling Kits, Biotium

Reactive CF® Dyes and Protein Labeling Kits, Biotium

Supplier: Biotium

Biotium offers a number of reactive dye formats for labelling proteins, nucleic acids, and other biomolecules with fluorescent dyes. CF® dyes offer advantages in brightness and photostability compared other similar commercial dyes. CF® dyes are available in a number of reactive dye formats for CF® dye colours span the visible and near-infrared spectra.

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Pierce™ EZ-Link™ Azide Reactive Biotinylation Reagents, Thermo Scientific

Pierce™ EZ-Link™ Azide Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce EZ-Link Phosphine-PEG3-Biotin is a biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.

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CF® 640R cAMP

Supplier: Biotium

Fluorescently labeled cAMP analogs that can be used to probe cAMP receptors.

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Streptavidin Conjugates, Biotium

Streptavidin Conjugates, Biotium

Supplier: Biotium

A high quality biotin-binding protein conjugated to Biotium's signature bright and photostable fluorescent CF® dyes, and a selection of other labels.

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HOOK™ Sulfhydryl Reactive Biotin Reagents and Kits for Highly Efficient Protein Labeling, G-Biosciences

HOOK™ Sulfhydryl Reactive Biotin Reagents and Kits for Highly Efficient Protein Labeling, G-Biosciences

Supplier: G-Biosciences

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

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CLIP-SURFACE® 488, New England Biolabs

CLIP-SURFACE® 488, New England Biolabs

Supplier: New England Biolabs (NEB)

CLIP-Surface™ 488 is a photostable green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells or in vitro

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Reactive Quencher Dyes, Biotium

Supplier: Biotium

Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.

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Alpha-Bungarotoxin, CF® Dye and Other Conjugates, Biotium

Alpha-Bungarotoxin, CF® Dye and Other Conjugates, Biotium

Supplier: Biotium

Conjugates of alpha-bungarotoxin labeled with a selection of our CF® dyes and other labels. Labeled alpha-bungarotoxin conjugates can be used for staining nicotinic acetylcholine receptors at neuromuscular junctions in tissue sections.

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Pierce™ NHS-Fluorescein Antibody Labeling Kit, Thermo Scientific

Pierce™ NHS-Fluorescein Antibody Labeling Kit, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ NHS-Fluorescein Antibody Labeling Kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

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SNAP-SURFACE® 549 , New England Biolabs

SNAP-SURFACE® 549 , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Surface™ 549 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

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Pierce™ EZ-Link™ Maleimide Activated Horseradish Peroxidase Kit, Thermo Scientific

Pierce™ EZ-Link™ Maleimide Activated Horseradish Peroxidase Kit, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

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SNAP-SURFACE® Alexa Fluor 546 , New England Biolabs

SNAP-SURFACE® Alexa Fluor 546 , New England Biolabs

Supplier: New England Biolabs (NEB)

SNAP-Surface Alexa Fluor 546 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

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Pierce™ Dye Removal Columns, Thermo Scientific

Pierce™ Dye Removal Columns, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

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EZ-Link™ Micro NHS-PEG4 Biotinylation Kit

EZ-Link™ Micro NHS-PEG4 Biotinylation Kit

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

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