For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.

• Highly purified recombinant enzyme
• Excellent batch-to-batch reproducibility
• Each lot is tested to be free of nuclease contamination

Taq DNA Polymerase (cloned) is the recombinant form of the native enzyme from Thermus aquaticus expressed in E. coli. Like native Taq, it polymerizes DNA from a primer annealed to a DNA template in the presence of deoxyribonucleotide triphosphates. Because Taq DNA Polymerase (cloned) is a recombinant protein, it is exceptionally pure and provides excellent batch-to-batch reproducibility. It has an optimum temperature of 75 °C and can survive repeated incubations at >95 °C.

Unit definition: One unit incorporates 10 nmol of total nucleotides into acid-insoluble material in 30 minutes at 70 °C in a total volume of 50 μl.

Storage conditions: –20 °C, 50 mM Tris-HCl, pH 7.5, 5 mM DTT, 0.1 mM EDTA, 50% Glycerol, Stabilizers