You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
HOOK™ Activated Agarose (Amine Reactive) for Affinity Column Generation, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups
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Recombinant Streptavidin, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' recombinant streptavidin comes from streptomyces avidinii, produced in E.coli
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Peptide Coupling Kits, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' HOOK™ Peptide Coupling (Amine Reactive) Kit is designed for the coupling of peptides to carrier proteins, utilizing the primary amines and carboxyl groups of the peptides and carrier proteins
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Pierce™ PEGylated SMCC Heterobifunctional Crosslinkers, Thermo Scientific
Supplier: Thermo Scientific
Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.
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Pierce™ NHS-Azide and NHS-Phosphine Reagents, Thermo Scientific
Supplier: Thermo Scientific
The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.
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Pierce™ DyLight™ Antibody Labeling Kits, Thermo Scientific
Supplier: Thermo Scientific
The DyLight™ Antibody Labeling Kits and were specifically developed for fast, efficient labeling of antibodies
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Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific
Supplier: Thermo Scientific
The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers
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SNAP-CELL® Block , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Cell® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag® in solution or in living cells
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CLIP-Biotin, New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Biotin is a cell-permeable substrate (BC-Biotin) based on biotin with an amidocaproyl linker
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Pierce™ Reduce-Imm™ Reducing Column, Thermo Scientific
Supplier: Thermo Scientific
Eliminate soluble components and reduce disulfide bonds without sacrificing valuable protein or peptide samples.
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IMPACT™ Kit for Protein Purification, New England Biolabs
Supplier: New England Biolabs (NEB)
The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.
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Pierce™ EZ-Link™ Plus Activated Peroxidase, Thermo Scientific
Supplier: Thermo Scientific
Activated peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins.
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SNAP-SURFACE® Block , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells
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Calmodulin Resin for CBP Purification, G-Biosciences
Supplier: G-Biosciences
The Calmodulin Resin Kit is for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.
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Streptavidin, New England Biolabs
Supplier: New England Biolabs (NEB)
A 52.8 kDa non-glycosylated protein with a very high affinity for biotin
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Pierce™ aminoxyTMTsixplex™ Label Reagent Set, Thermo Scientific
Supplier: Thermo Scientific
The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) label reagents enable multiplexed characterization and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidized proteins) by mass spectrometry (MS).
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Pierce™ Sulfhydryl Addition Kit, Thermo Scientific
Supplier: Thermo Scientific
Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.
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HOOK™ Carboxyl Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences
Supplier: G-Biosciences
The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research
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CLIP-SURFACE® 647, New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Surface™ 647 is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells, or in vitro
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Pierce™ iodoTMTsixplex™ Isobaric Label Reagent Set, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific iodoTMTsixplex Label Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry.
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SNAP-CELL® 430 , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Cell® 430 is a blue fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro
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Pierce™ Fluorescence Biotin Quantitation Kit, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific™ Pierce Fluorescence Biotin Quantitation Kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.
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Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific
Supplier: Thermo Scientific
DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.
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SNAP-TAG® Purified Protein, New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates
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Pierce™ Controlled Protein-Protein Cross-Linking Kit, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.They insert an unbreakable molecular bridge between two proteins with easy-to-control stepwise coupling.
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femtoCHROMO™- AP Kit for Chromogenic Detection, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' femtoCHROMO™- AP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining
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Pierce™ Azide Labeled Sugars, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce™ Azide-Labeled sugars provide a highly specific approach for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.
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Pierce™ Fluor Phosphine Reagents, DyLight™, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific DyLight Fluor Phosphine Reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.
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SNAP-SURFACE® Alexa Fluor 647 , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Surface Alexa Fluor 647 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells
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Pierce™ EZ-Link™ Amine Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Thermo Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.