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Specifications
- Description:Ready-To-Go T-Primed First-Strand Kit
- Cat. no.:CA95040-332L
- No. of Reactions:50
Specifications
About this item
Ready-To-Go™ T-Primed First-Strand Kit has been designed to provide all the reagents necessary to generate full length first-strand cDNA from an mRNA template using an oligo(dT) primer containing a Not I restriction site. The components in the kit are provided in a room-temperature-stable format such that after reconstitution, each first-strand reaction mix is ready for immediate use in a first strand cDNA reaction. First-strand cDNA generated with this kit can be used as a template for standard Gubler-Hoffman second-strand cDNA synthesis or for amplification by PCR.
- Pre-formulated, ambient-temperature-stable, single-dose glassified reaction mixes minimise pipetting errors, avoid potential cross-contamination of subsequent PCR products, and ensure optimal performance from each reaction
- Predispensed reaction mixes are highly suitable for research applications which use PCR to detect and quantify eukaryotic RNA species isolated from a variety of cell sources and tissue samples
- Reaction mixes include NotI-(dT)₁₈ primer in sufficient quantity to serve as a downstream PCR primer for performing 3'-RACE
- Final reaction volume is 33 µl once reconstituted