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Specifications
- Size:96 Wells
- Environmentally Preferable:
- Storage conditions:–80 °C (Long Term)
- Application:Fluorescent Detection, HTS Activity Assay, Cell-based Assays
- Cat. no.:89156-522
- Supplier No.:AK555-0001
Specifications
About this item
A FLUOR DE LYS® fluorescent assay system
- Useful for inhibitor screening or characterizing enzyme kinetics
- Includes optimal substrate selected from a panel of acetylated sites in p53 and histones
The Fluor de Lys® SIRT1 assay is based on the Fluor de Lys® SIRT1 Substrate and Fluor de Lys® Developer II combination. The assay procedure has two steps. First, the Fluor de Lys® SIRT1 Substrate, which contains a peptide comprising amino acids 379-382 of human p53 (Arg-His-Lys-Lys(Ac)). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer II produces a fluorophore.
The SIRT1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT1 included in the kit.