This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites.

Immunoprecipitation; Western Blot (20µl/lane (mini gel) for HRPO/DAB detection, or 5µl/lane (mini gel) for HRPO/ECL detection). Do not use milk/casein for blocking and dilution! Replace by BSA! We recommend to use 1% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 3500, 0.2% Tween 20 in 2x PBS.Optimal conditions must be determined individually for each application.

Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.