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202 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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CF®488A Alkyne

CF®488A Alkyne

Supplier: Biotium

CF® Dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.

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Biotin-20-dUTP, Biotium

Biotin-20-dUTP, Biotium

Supplier: Biotium

Biotin-20-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.

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Biotin-16-dUTP, Biotium

Biotin-16-dUTP, Biotium

Supplier: Biotium

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.

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Annexin V, CF® 770 conjugate

Annexin V, CF® 770 conjugate

Supplier: Biotium

Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.

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CF™ Dye Streptavidin and Monoclonal Mouse Anti-Biotin Conjugates, Biotium

Supplier: Biotium

Biotium offers a variety of streptavidin and anti-biotin antibody conjugates featuring our series of CF dyes

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R-Phycoerythrin (RPE), Columbia Biosciences

R-Phycoerythrin (RPE), Columbia Biosciences

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin

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B-Phycoerythrin (BPE), Columbia Biosciences

B-Phycoerythrin (BPE), Columbia Biosciences

Supplier: Columbia Biosciences

Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore

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Colorimetric and Fluorogenic Enzyme Substrates, Biotium

Supplier: Biotium

Popular chromogenic and fluorogenic substrates for phosphatases, peroxidases, and other enzymes.

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dUTP CF® Dye Conjugates, Biotium

Supplier: Biotium

CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.

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Mix-n-Stain™ Antibody Labeling Kits, Biotium

Mix-n-Stain™ Antibody Labeling Kits, Biotium

Supplier: Biotium

Mix-n-Stain™ antibody labeling kits dramatically simplify the process of labeling primary antibodies with fluorescent dyes or biotin

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CF™ Dye Bovine Serum Albumin Conjugates, Biotium

Supplier: Biotium

Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important

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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates, Biotium

CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates, Biotium

Supplier: Biotium

Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.

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ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit

ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST INC

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Biotin-11-dUTP, Biotium

Biotin-11-dUTP, Biotium

Supplier: Biotium

Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling.

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CF®405S BCN

CF®405S BCN

Supplier: Biotium

CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.

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Allophycocyanin (Crosslinked APC), Columbia Biosciences

Allophycocyanin (Crosslinked APC), Columbia Biosciences

Supplier: Columbia Biosciences

Allophycocyanin (APC) is a commonly used label in Time-Resolved Fluorescence Resonant Energy Transfer (TR-FRET) assays for High Throughput Screening and Flow Cytometry.

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ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit

Supplier: AAT BIOQUEST INC

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

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Thiol-Reactive Fluorescent Dyes and Other Thiol-Modifying Reagents, Biotium

Thiol-Reactive Fluorescent Dyes and Other Thiol-Modifying Reagents, Biotium

Supplier: Biotium

MTS, TS, and maleimide, and other thiol-modifying reagents and reactive fluorescent dyes.

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ExoBrite™ STORM CTB EV Staining Kits, Biotium

ExoBrite™ STORM CTB EV Staining Kits, Biotium

Supplier: Biotium

Fluorescent cholera toxin subunit B (CTB) conjugates that are optimized for direct stochastic optical reconstruction microscopy (dSTORM) imaging of EVs and exosomes.

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D-Luciferin Luminescent Substrates, Biotium

Supplier: Biotium

Luciferin is a substrate for the enzyme firefly luciferase

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HRP WGA Wheat Germ Agglutinin (WGA) Conjugates

HRP WGA Wheat Germ Agglutinin (WGA) Conjugates

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

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VivoBrite™ rapid antibody labeling kit for small animal in vivo imaging, CF™790 SE

VivoBrite™ rapid antibody labeling kit for small animal in vivo imaging, CF™790 SE

Supplier: Biotium

Near-infrared dyes offer highly sensitive and specific detection in complex biological systems, because cells and tissues have intrinsically low autofluorescence in the near-IR region

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Lipophilic Carbocyanine Dyes and CellBrite Cytoplasmic Membrane Labeling Kits, Biotium

Supplier: Biotium

Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes

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Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits, Biotium

Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits, Biotium

Supplier: Biotium

Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium

Coelenterazine Substrates for Renilla Luciferase and Apoaequorin, Biotium

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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CF®DI Aminooxy, Biotium

Supplier: Biotium

CF®DI aminooxy dyes react with aldehyde/ketone groups on proteins or other biomolecules to form a stable oxime bond. The CF®DI dyes (CF®488DI, CF®555DI and CF®647DI) are designed to match each other for molecular weight and charge. The molecular weights of the same protein conjugated to different CF®DI dye colors are close to each other and the pl of the labeled proteins remains the same.

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Reactive CF® Dyes and Protein Labeling Kits, Biotium

Reactive CF® Dyes and Protein Labeling Kits, Biotium

Supplier: Biotium

Biotium offers a number of reactive dye formats for labelling proteins, nucleic acids, and other biomolecules with fluorescent dyes. CF® dyes offer advantages in brightness and photostability compared other similar commercial dyes. CF® dyes are available in a number of reactive dye formats for CF® dye colours span the visible and near-infrared spectra.

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HOOK™ Photoreactive Biotin Reagent for Highly Efficient Protein Labeling, G-Biosciences

HOOK™ Photoreactive Biotin Reagent for Highly Efficient Protein Labeling, G-Biosciences

Supplier: G-Biosciences

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

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Pierce EZ-Link® Photo Reactive Biotinylation Reagents, Thermo Scientific

Pierce EZ-Link® Photo Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Thermo Scientific

Photoactivatable Biotin, Biotin-LC-ASA, Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups.

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HOOK™ Carbohydrate Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences

HOOK™ Carbohydrate Reactive Biotin Reagents & Kits for Highly Efficient Protein Labeling, G-Biosciences

Supplier: G-Biosciences

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

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