Specifications
- Protein/peptide type:Recombinant
- Species:Mouse
- Size:10 µg
- Endotoxin content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
- Reconstitution instructions:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
- Protein/peptide name:TNC:TWEAK (soluble)
- Purity:>95% (SDS-PAGE)
- Concentration:0.1mg/ml after reconstitution.
- Formulation:Lyophilized. Contains PBS.
- Cat. no.:89151-948
- Supplier No.:522-138-C010
Specifications
About this item
Produced in HEK 293 cells. The extracellular domain of mouse TWEAK (aa 94-249) is fused at the N-terminus to tenascin-C (TNC) (chicken) (aa 110-139) and a FLAG®-tag.
Some TNF ligands (such as TWEAK (mouse) or FasL (mouse)) produced as soluble forms do not assemble into stable trimers and show low receptor binding. Addition of the tenascin-C oligomerization domain (TNC) (from chicken) at the N-terminus of recombinant mouse soluble TWEAK stabilizes the TNF homology domain (THD) of rmsTWEAK into a trimer and increases binding to its respective TNF receptor. Note: For some TNF ligands, such as FasL (mouse), activated by further oligomerization (> hexamer), the presence of TNC is not sufficient and ligand crosslinking using the "Enhancer for Ligands" (Prod. No. ALX-804-034) is required.