Human Recombinant TNF alpha (soluble) (from E. coli)

89151-692

: Enzo Life Sciences

89151-692

: Enzo Life Sciences

: 522-008-C050

Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Size:
50 µg
Endotoxin content:
<0.1EU/µg purified protein (LAL test; Bio Whittaker).
Biological activity:
Exerts its biological activity in a concentration range of 0.1-1ng/ml (WEHI 164 cells).
ED50: 0.1ng/ml (WEHI 164 cells)
Reconstitution instructions:
Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Protein/peptide name:
TNF alpha (soluble)
Purity:
>95% (SDS-PAGE)
Concentration:
1mg/ml after reconstitution.
Formulation:
Lyophilized. Contains PBS.
Cat. no.:
89151-692
Supplier No.:
522-008-C050

Produced in E. coli. The extracellular domain of human TNF-α (tumor necrosis factor-α) (aa 85-223) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.