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Specifications
- Antibody type:Secondary
- Antigen name:Protein A
- Conjugation:RPE (R-Phycoerythrin)
- Storage buffer:BSA 10 mg/ ml, PBS 0.05 % azide.
- Storage temperature:2-8 °C in dark
- Cat. no.:89352-756
- Supplier No.:GTX30957
Specifications
About this item
Protein A (MW 42 kDa) is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. One protein A molecule has been shown to bind at least 2 molecules of IgG simultaneously. The IgG binding domain of Protein A consists of three anti-parallel α-helicies, the third of which is disrupted when the protein is complexed with the Fc region of the immunoglobulins. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a, and IgG2b. Protein A also binds IgGs from other species, including monkey, rabbit, pig, guinea pig, dog, and cat. Phycoethyrin: Phycoethyrin (R-PE) is a 240 kDa protein from the light-harvesting phycobilliprotein, phycoerythrin is composed of a protein part, organised in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. In the phycoerythrin family, the phycobilins are: phycoerythrobilin, the typical phycoerythrin acceptor chromophore, and sometimes phycourobilin (marine organisms). Phycoerythrins are the phycobilliprotein which bind the highest number of phycobilins (up to six per alpha-beta subunit dimer). R-PE purified from Poryhyra tenera; A565/A280 >5.0; A 1% 565 nm=82; Amax = 490, 545 and 566 nm; E max = 580 nm.
Type: Secondary
Antigen: Protein A
Clonality:
Clone:
Conjugation: R-PE
Epitope:
Host:
Isotype:
Reactivity: