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Specifications
- Antibody type:Primary
- Antigen name:Green Fluorescence Protein
- Clonality:Monoclonal
- Host:Mouse
- Isotype:IgG2a
- Antigen symbol:GFP
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Antigen synonyms:GFPuv,eGFP,Green Fluorescent Protein
- Storage buffer:Ascitic fluid containing 0.03% sodium azide.
- Molecular weight:27kDa
- Storage temperature:Store at 4℃, for long term storage, store at -20℃.
- Immunogen:Purified recombinant fragment of GFP expressed in E. Coli.
- Tested applications:Fluorescence-Activated Cell Sorting
- Cat. no.:89353-898
Specifications
About this item
GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Type: Primary
Antigen: GFP
Clonality: Monoclonal
Clone:
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Other