To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Antibody type:Primary
- Antigen name:Coronin 1A
- Antigen symbol:CORO1A
- Clonality:Polyclonal
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Goat
- Isotype:IgG
- Reactivity:Bovine
- Western blot:Yes
- Size:50 μg
- Antigen synonyms:HCORO1|IMD8|p57|CLABP|CLIPINA|TACO
- Gene ID:11151
- Concentration:0.5 mg/ml
- Immunogen:Synthetic peptide: KRLDRLEETVQAK, corresponding to amino acids 449-461 of Human Coronin 1.
- Purification:Immunogen affinity purified
- Storage buffer:Preservative: 0.02% Sodium Azide; Constituents: 0.5% BSA, 0.5mg/ml Tris-saline. pH 7.3
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Tested applications:ELISA, WB
- Supplier No.:GTX14787
Coronin 1 was first isolated from Dictyostelium, but similar proteins have been identified in many species and individual cell types. The coronin-like protein in yeast promotes actin polymerization and also interacts with microtubules. Dictyostelium mutants lacking coronin are impaired in cytokinesis and all actin-mediated processes. Analysis of coronin-GFP (green-fluorescent protein) fusions and knockout mutants shows that coronin participates in the remodelling of the cortical actin cytoskeleton that is responsible for phagocytosis and macropinocytosis. Likewise, in mammalian neutrophils, a coronin-like protein is also associated with the phagocytic apparatus. Coronin 1 binds actin (by similarity) and is found in noninfected macrophages, localised to the cortical microtubule network; in mycobacteria-infected macrophages, progressively relocalized and retained around the mycobacterial phagosomes. Coronin1/TACO is expressed in brain, thymus, spleen, bone marrow and lymph node. Low expression observed in lung and gut.
Peptide ELISA: Use at a dilution of 1/16000. WB: Use at a concentration of 0.25 - 1 ?g/ml. Detects a band of approximately 60 kDa in Molt-4 and Jurkat lysates (predicted molecular weight: 53 kDa according to NP_009005). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: CORO1A
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype: IgG
Reactivity: Human, Mouse, Dog, Rat, Bovine