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Specifications
- Antibody type:Primary
- Antigen name:Calpain 1
- Clonality:Monoclonal
- Clone:15C10
- Gene ID:823
- Host:Mouse
- Isotype:IgG1
- Reactivity:Bovine
- Antigen symbol:CAPN1
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoPrecipitation:Yes
- Western blot:Yes
- Antigen synonyms:muCANP,CANP,CANPL1,CANP1,muCL
- Storage buffer:50% Glycerol, 0.15M Sodium chloride, 0.02M Sodium phosphate. pH 7.5 containing 3mM Sodium azide
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Full length human protein
- Tested applications:ELISA, IP, WB
- Purification:Protein G purified
- Cat. no.:89367-470
Specifications
About this item
Mu Calpain, also known as Calpain I, and m calpain, also known as Calpain II, are intracellular, calcium-dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. The calpains have papain-like activity, thus the -pain nomenclature. Both m calpain and mu calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV.
For ELISA: Use at a concentration of 0.5-1 ?g/ml. IP: Use at a concentration of 1-2 ?g/ml. For WB: Use at a concentration of 0.5-1 ?g/ml. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: CAPN1
Clonality: Monoclonal
Clone: 15C10
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Bovine