You searched for: Western Blotting Reagents
Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Amersham™ ECL™ Western Blotting Detection Reagent, Cytivia
Supplier: Cytiva
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
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Amersham ECL Western Blotting Select Detection Reagent, Cytiva
Supplier: Cytiva
Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.
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Western blotting detection reagent, Amersham™ ECL™ Prime
Supplier: Cytiva
Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.
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Protein Free Blot Blocking Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure Protein Free Blot Blocking Buffer is a protein free blocking solution, optimized for use with fluorescent Western blots, and also an excellent blocking reagent for chemiluminescent Western blotting.
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Amersham ECL Western Blotting Start Detection Reagents, Cytiva
Supplier: Cytiva
Chemiluminescence detection reagent for detection of high to medium abundant proteins.
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Fluorescent Wash Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure IR Fluorescent Blot Washing Buffer is optimized for use with visible and infrared fluorescent Western blotting.
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Radiance Plus Chemiluminescent Substrate, Azure Biosystems
Supplier: Azure Biosystems
Radiance Plus is the most sensitive HRP substrate for chemiluminescent western blotting available from Azure.
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Radiance Q Chemiluminescent Substrate, Azure Biosystems
Supplier: Azure Biosystems
Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability
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Transfer Buffer, Azure Biosystems
Supplier: Azure Biosystems
Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.
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Amersham ECL Western Blotting Blocking Agent, Cytiva
Supplier: Cytiva
Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents
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ECL Western Blotting ECF Substrate, Cytiva
Supplier: Cytiva
Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.
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Pierce Miser™ Western Blotting Detection Reagents, Antibody Extender Solution NC, Thermo Scientific
Supplier: Thermo Scientific
Pierce Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.
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Amersham ECL Gold Hybridization Buffer, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
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Western blotting system, Amersham ECL™
Supplier: Cytiva
Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.
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Fluorescent Labeling Reagents, CypHer5E NHS Ester, Cytiva
Supplier: Cytiva
CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.
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Pharmalytes® IEF Carrier Ampholytes, Cytiva
Supplier: Cytiva
Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
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Cy™ Amidites
Supplier: Cytiva
Cy™ fluorescent amidites for incorporation of Cy3, Cy3.5, Cy5, Cy5.5 fluorescent dyes at any position in an oligonucleotide during synthesis.
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Nucleic acid labelling and Detection System, Amersham ECL Direct™
Supplier: Cytiva
ECL Direct™ nucleic acid labelling and detection systems are based on the direct labelling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalysed breakdown of luminol. Suitable for fast and easy detection of medium to high target amounts in applications such as colony/plaque screens, dot blots and PCR product analyses.
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Amersham ECL Plex Western Blotting Combination Packs, Cytiva
Supplier: Cytiva
Amersham ECL Plex™ Western blotting combination packs offer a complete system for fluorescent Western blotting, with improved formulation Cy3 conjugates for Lower background.
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Amersham™ ECL™ Prime Blocking Reagent
Supplier: Cytiva
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
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Acridinium Ester, (Trigger Solution), Enzo Life Sciences
Supplier: Enzo Life Sciences
Species Independent
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Amersham ECL Direct Labeling System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
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DeStreak Rehydration Reagent, Cytiva
Supplier: Cytiva
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.
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HL60 Cell Extract (Non-induced), Enzo Life Sciences
Supplier: Enzo Life Sciences
Whole-cell extract of human HL-60 leukemia cells.
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CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva
Supplier: Cytiva
CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.
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Restore™ Fluorescent Western Blot Stripping Buffer, Thermo Scientific
Supplier: Thermo Scientific
The Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.
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Nucleic acid labelling and detection system, AlkPhos Direct™
Supplier: Cytiva
AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).
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Amersham ECL Direct Labeling and Detection System, Cytiva
Supplier: Cytiva
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
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Pierce™ Fast Western Blot Kit, ECL Substrate, Thermo Scientific
Supplier: Thermo Scientific
The Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.
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Genomic DNA, Mouse, EMD Millipore
Supplier: MilliporeSigma
Genomic DNAs are highly purified preparations of high molecular weight genomic DNA from a variety of organisms.