You searched for: Western Blotting Reagents

Supplier: Cytiva

Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

Supplier: Cytiva

Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

Supplier: Azure Biosystems

Azure Protein Free Blot Blocking Buffer is a protein free blocking solution, optimized for use with fluorescent Western blots, and also an excellent blocking reagent for chemiluminescent Western blotting.

Supplier: Cytiva

Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Supplier: Cytiva

Chemiluminescence detection reagent for detection of high to medium abundant proteins.

Supplier: Azure Biosystems

Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability

Supplier: Azure Biosystems

Azure IR Fluorescent Blot Washing Buffer is optimized for use with visible and infrared fluorescent Western blotting.

Supplier: Azure Biosystems

Radiance Plus is the most sensitive HRP substrate for chemiluminescent western blotting available from Azure.

Supplier: Azure Biosystems

Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

Supplier: Cytiva

Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents

Supplier: Thermo Scientific

Pierce Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

Supplier: Abcam

The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots.

Supplier: Cytiva

Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.

Supplier: ABCAM INC CA

Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy.

Supplier: ABCAM INC CA

NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting.

Supplier: ABCAM INC CA

Rapid DAB Detection System (ab290116) is a powerful Intensification system that significantly enhances chromogenic signals.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

Supplier: ABCAM INC CA

A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

Supplier: ABCAM INC CA

High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band.

Supplier: ABCAM INC CA

Avidin/biotin blocking kit blocks signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection. - Cited in over 35 publications - Ideal for tissues rich in biotin (e.g., liver, kidney) - Easy-to-use, quick protocol

Supplier: Cytiva

ECL Direct™ nucleic acid labelling and detection systems are based on the direct labelling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalysed breakdown of luminol. Suitable for fast and easy detection of medium to high target amounts in applications such as colony/plaque screens, dot blots and PCR product analyses.

Supplier: ABCAM INC CA

This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies.

Supplier: ABCAM INC CA

Antibody diluent (pH 7 - 7.8) is used to dilute primary antibodies to their working concentrations for use in immunohistochemistry (IHC). - Cited in over 15 publications - Reduces background and non-specific binding - Extends antibody stability up to 24 months at 2-8°C - Tris-buffered with stabilizing protein, wetting agent, antimicrobial agent, and green dye

Supplier: ABCAM INC CA

The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.

Supplier: ABCAM INC CA

Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.

Supplier: Cytiva

Cy™ fluorescent amidites for incorporation of Cy3, Cy3.5, Cy5, Cy5.5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Supplier: Cytiva

Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Supplier: ABCAM INC CA

AEC substrate kit for use in IHC using HRP / peroxidase detection.

Supplier: Cytiva

CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.