You searched for: Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

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Pierce™ Immobilized Papain (Agarose Resin), Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce Immobilized Papain consists of a cysteine-endopeptidase (papain) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.

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Factor Xa Protease, New England Biolabs

Supplier: New England Biolabs (NEB)

Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg

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Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.

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Pierce™ Endoproteinase Glu-C Protease, MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Glu-C Protease, MS Grade, is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications, in-solution digestion of proteins and in-gel digestion of proteins. Store at -20 ˚C in a nonfrost-free freezer.

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Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific

Supplier: Thermo Scientific

Immobilized Pepsin agarose consists of an acidic endopeptidase that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.