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35 results for Protein Extraction Reagents

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Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

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Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.

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FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer II Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.

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SMART Digest™ Protein Digests Kits

SMART Digest™ Protein Digests Kits

Supplier: Thermo Scientific

Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.

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FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

FLUOR DE LYS® Developer I Concentrate, Enzo Life Sciences

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.).

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Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific

Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific

Supplier: Thermo Scientific

Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques.

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Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific

Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.

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BugBuster® HT Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster® HT Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E

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BugBuster® Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster® Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E.coli, resulting in the liberation of soluble protein.

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VersaLyse™ Lysing Solution, 100 Tests, RUO

Supplier: Beckman Coulter

VersaLyse Lysing solution is a reagent used to lyse red blood cells from any biological fluid and, in particular, to lyse erythrocytes from whole blood. VersaLyse is a highly specific, very gentle lysing solution, suitable for applications such as immunophenotyping, cell sorting, activation or cell culture. It is designed for use in flow cytometry and facilitates obtaining highly discriminative scattergrams whatever the type of flow cytometer used. VersaLyse is a versatile reagent which may be used in lyse/wash, wash/lyse and lyse/no-wash procedures with undiluted or diluted samples. The histograms are dual-parameter light scatter histograms (FS versus SS) of a normal K3EDTA anticoagulated blood sample lysed with VersaLyse Lysing Solution (No-wash procedure).

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PopCulture® Reagent, MilliporeSigma

Supplier: MilliporeSigma

PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.

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BugBuster® 10X Protein Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of salts or buffer components.

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BugBuster® (primary amine-free) Extraction Reagent, MilliporeSigma

Supplier: MilliporeSigma

BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking.

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Lysonase™ Bioprocessing Reagent, EMD Millipore

Supplier: MilliporeSigma

Lysonase™ bioprocessing reagent is an optimized, ready-to-use blend of rLysozyme™ solution and benzonase nuclease.

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Lysing Matrix Z, MP Biomedicals

Lysing Matrix Z, MP Biomedicals

Supplier: MP Biomedicals

Lysing Matrix Z is used for breaking tough plant and animal samples.

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NP-40 Alternative, MilliporeSigma

Supplier: MilliporeSigma

NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.

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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific

Supplier: Thermo Scientific

B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

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Q-sep® QuEChERS Extraction Salts, Restek

Q-sep® QuEChERS Extraction Salts, Restek

Supplier: Restek

QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.

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Pepsin Reagent, Ready To Use, Electron Microscopy Science

Supplier: Electron Microscopy Sciences

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Pierce™ Trypsin Protease, MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling

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Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific

Supplier: Thermo Scientific

Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.

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Iminobiotin Resin for Isolation of Avidin, Streptavidin and Neutravidin Complexes, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose

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Protein Reducing Agents, G-Biosciences

Protein Reducing Agents, G-Biosciences

Supplier: G-Biosciences

A water soluble, odorless, non-toxic and stable protein reductant, G-Biosciences' Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to ß-mercaptoethanol and DTT (dithiothreitol)

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Pierce™ Detergent Removal Spin Column, Thermo Scientific

Pierce™ Detergent Removal Spin Column, Thermo Scientific

Supplier: Thermo Scientific

Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.

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Biotin Resin for Isolation of Avidin, Streptavidin & Neutravidin Coupled Molecules, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.

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Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific

Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.

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ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Factor Xa Protease, New England Biolabs

Factor Xa Protease, New England Biolabs

Supplier: New England Biolabs (NEB)

Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-(Glu or Asp)-Gly-Arg

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Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific

Pierce™ Endoproteinase Asp-N Protease, MS Grade, Thermo Scientific

Supplier: Thermo Scientific

Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications. Store at -20˚C in a nonfrost-free freezer.

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Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Supplier: Thermo Scientific

The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.

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