Protein Extraction Reagents
Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.
Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.
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Pierce™ Immobilized Pepsin (Agarose Resin), Thermo Scientific
Supplier: Thermo Scientific
Immobilized Pepsin agarose consists of an acidic endopeptidase that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.
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PopCulture® Reagent, MilliporeSigma
Supplier: MilliporeSigma
PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium.
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BugBuster® HT Protein Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster® HT Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E
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NP-40 Alternative, MilliporeSigma
Supplier: MilliporeSigma
NP-40 alternative is a non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis.
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BugBuster® (primary amine-free) Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking.
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BugBuster® 10X Protein Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of salts or buffer components.
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BugBuster® Protein Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
BugBuster® Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E.coli, resulting in the liberation of soluble protein.
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Lysonase™ Bioprocessing Reagent, EMD Millipore
Supplier: MilliporeSigma
Lysonase™ bioprocessing reagent is an optimized, ready-to-use blend of rLysozyme™ solution and benzonase nuclease.
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Lysing Matrix Z, MP Biomedicals
Supplier: MP Biomedicals
Lysing Matrix Z is used for breaking tough plant and animal samples.
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Pierce™ Surfact-Amps™ Detergent Solutions, Thermo Scientific
Supplier: Thermo Scientific
Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques.
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B-PER™ Pierce™ Complete Bacterial Protein Extraction Reagent, Thermo Scientific
Supplier: Thermo Scientific
B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.
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Pierce™ Endoproteinase Lys-C Protease, MS Grade, Thermo Scientific
Supplier: Thermo Scientific
Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
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SMART Digest™ Protein Digests Kits
Supplier: Thermo Scientific
Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits.
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Universal HIER antigen retrieval reagent (10X), Abcam
Supplier: Abcam
Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers. - Cited in over 20 publications - Colour-coded pH indicator for accuracy - Superior pH stability at high temperatures - Non-toxic, odorless, and azide-free - Optimized for FFPE tissue antigen retrieval
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Pepsin Reagent, Ready To Use, Electron Microscopy Science
Supplier: Electron Microscopy Sciences
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
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