You searched for: Cloning Kits and Ligation

Supplier: New England Biolabs (NEB)

This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

Supplier: TELESIS BIO

Generate seamless plasmid DNA or BAC clones without restriction digestion.

Supplier: TELESIS BIO

The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp® 3250.

Supplier: New England Biolabs (NEB)

This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

Supplier: TELESIS BIO

The Select DNA cloning Golden Gate Assembly BsmBI kits are compatible with the Type IIS restriction enzymes BsmBI. Please choose the appropriate kit based on the number of fragments being assembled.

Supplier: TELESIS BIO

BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.

Supplier: TELESIS BIO

The Select DNA cloning Gibson assembly kits can generate Gibson clones starting with fragments and vector supplied by the end user.

Supplier: TELESIS BIO

Generate seamless plasmid DNA or BAC clones without restriction digestion.

Supplier: TELESIS BIO

The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp 9600.

Supplier: TELESIS BIO

BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.

Supplier: TELESIS BIO

The Select DNA cloning golden gate assembly, BsaI kits are compatible with the Type IIS restriction enzymes BsaI. Please choose the appropriate kit based on the number of fragments being assembled.

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

Supplier: Cytiva

T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Supplier: MilliporeSigma

The pT7Blue cloning kit features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.

Supplier: Lucigen

The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This enzyme catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA and efficiently joins blunt and cohesive ends, and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Supplier: New England Biolabs (NEB)

The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments

Supplier: New England Biolabs (NEB)

The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature

Supplier: New England Biolabs (NEB)

The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E

Supplier: New England Biolabs (NEB)

NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly

Supplier: New England Biolabs (NEB)

NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly.

Supplier: New England Biolabs (NEB)

Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Supplier: New England Biolabs (NEB)

Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends.

Supplier: New England Biolabs (NEB)

T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi

Supplier: New England Biolabs (NEB)

The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors

Supplier: New England Biolabs (NEB)

ElectroLigase™ combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG.

Supplier: New England Biolabs (NEB)

9°N™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA