You searched for: Nucleic Acid Reagents

Supplier: New England Biolabs (NEB)

PvuI-HF® is produced in E. coli from a strain that carries the cloned and modified PvuI gene from Proteus vulgaris (ATCC 13315).

Supplier: New England Biolabs (NEB)

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. Unique Dual Index (UDI) primer pairs enable the identification and mitigation of the impacts of index hopping when using a patterened flow cell.

Supplier: New England Biolabs (NEB)

Phusion Hot Start Flex 2X Master Mix offers robust high fidelity performance and room temperature reaction setup in a convenient master mix format

Supplier: New England Biolabs (NEB)

OneTaq® Hot Start 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep VentR ™ DNA Polymerases combined with an aptamer-based inhibitor.

Supplier: New England Biolabs (NEB)

HindIII-HF® is an E. coli strain that carries the cloned and modified HindIII gene from Haemophi­lus influenzae Rd (ATCC 51907). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.

Supplier: Corning

The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilizes a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimized buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Supplier: New England Biolabs (NEB)

An E.coli strain that carries a TaqαI overproducing plasmid (F. Barany using an NEB clone). TaqαI has a two amino acid replacement at its amino terminus. This allows for a higher level of expression without interfering with its catalytic properites.

Supplier: New England Biolabs (NEB)

For optimal workflow flexibility, NEBNext® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina® library production.

Supplier: New England Biolabs (NEB)

OneTaq® Quick-Load® 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep VentR ™ DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products.

Supplier: G-Biosciences

G-Biosciences' OmniTemplate™ is specifically designed for the rapid isolation of a DNA template for polymerase chain reaction (PCR) analysis from mammalian tissue samples, blood and cell cultures

Supplier: New England Biolabs (NEB)

The Monarch® RNA cleanup kit enables fast and simple purification and concentration of up to 10 µg of RNA from enzymatic reactions.

Supplier: New England Biolabs (NEB)

Immobilized T4 DNA Ligase (IM-T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg per ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, enzyme can be removed using a magnet thereby avoiding heat inactivation, and can be re-used.

Supplier: New England Biolabs (NEB)

The Taq PCR Kit contains a sufficient supply of recombinant, highly purified Taq DNA Polymerase, PCR-qualified buffer solutions, deoxynucleotides and a broad-range, pre-mixed, ready-to-load DNA marker to perform 200 PCR reactions.

Supplier: New England Biolabs (NEB)

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (CutSmart® Buffer).

Supplier: PROMEGA SUNNYVALE INC SE

Pre-mixed, and ready to use, this master mix contains DNA polymerase, dNTPs, MgCl₂, and reaction buffers at optimal concentrations for the amplification of DNA via PCR. Its special formulation allows for direct loading of PCR samples into agarose gels as well as tracking the migration during electrophoresis without the need for loading dyes. Simply add template, primers, and nuclease-free water, available separately. Mater Mix reduces risk of contamination. Includes enough for 100 reactions. Note: Can be stored at 4 °C for up to six weeks, or −20 °C for longer.

Supplier: Thermo Scientific

The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.

Supplier: New England Biolabs (NEB)

The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA.

Supplier: Omega Bio-Tek

Isolate plasmid DNA from up to 5 ml culture in 9 minutes utilizing an innovative lysate clearance column.

Supplier: New England Biolabs (NEB)

An E. coli strain that carries the cloned and modified SpeI gene from Sphaerotilus species (ATCC 13923). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Supplier: Zymo Research

ZymoScript RT PreMix is an optimized reverse transcription kit containing all the necessary components for quick and efficient cDNA synthesis.

Supplier: New England Biolabs (NEB)

Monarch® Spin collection tubes are designed to be used with Monarch spin columns.

Supplier: Zymo Research

Prepare robust methyl-seq libraries in as little as three steps.

Supplier: IBI Scientific

IBI's Maxi Fast-Ion Plasmid Kits are endotoxin free and use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures

Supplier: New England Biolabs (NEB)

An E. coli strain that carries the cloned and modified SpeI gene from Sphaerotilus species (ATCC 13923). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Supplier: G-Biosciences

G-Biosciences' EZ Yeast™ Plasmid Prep kit is designed for the rapid isolation of 2µ plasmids from yeast patches or yeast grown in small liquid cultures

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the cloned and modified BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

Supplier: New England Biolabs (NEB)

The Monarch spin PCR and DNA cleanup kit (5 μg), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription.

Supplier: Beckman Coulter

DNA cleanup from enzymatic reactions. The EMnetik PCR Cleanup Kit is a revolutionary bead-based DNA cleanup kit. Using new SuperSPRI bead technology combined with the microparticle processor EMnetik 24, a user can complete PCR cleanups in 16 minutes.

Supplier: G-Biosciences

G-Biosciences' GET™ DNA Template is a spin column format kit suitable for the preparation of DNA templates from blood, cells, fungal and animal tissue samples

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The CRISPRclean® stranded total RNA prep with rRNA depletion (HMR + pan bacteria) is a total RNA library prep leveraging a CRISPR-powered workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples.