You searched for: Nucleic Acid Reagents
The wide range of reagents are suitable for use with nucleic acids in transfection and transformation procedures, as well as cloning, sequencing, purification, and extraction. Use these reagents for isolating and purifying nucleic acids from biological material for use in further laboratory procedures, and their ready-to-use status makes them easy and straightforward to apply to nucleic acids upon opening. Nucleic acid reagents are tailored to suit a number of different and varied applications.
ONETAQ® 2X Master Mix with Standard Buffer, New England Biolabs
Supplier: New England Biolabs (NEB)
OneTaq® 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep VentR™ DNA Polymerases ideally suited to routine PCR applications from a variety of templates including pure DNA solutions, bacterial colonies, and cDNA products
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TaqI-v2, New England Biolabs
Supplier: New England Biolabs (NEB)
An E.coli strain that carries a TaqαI overproducing plasmid (F. Barany using an NEB clone). TaqαI has a two amino acid replacement at its amino terminus. This allows for a higher level of expression without interfering with its catalytic properites.
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Restriction Enzymes
Supplier: MilliporeSigma
One unit of this Restriction Enzyme will digest 1{mu lower case}g of DNA in 60 minutes in 50mL r x n volume. Each comes in a recyclable packaging that holds the enzyme, buffers, and a lot-specific product information sheet that contains details of the rigorous quality control assays performed, product storage and usage information, protocols, and references.Note: All restriction enzymes require storage at -20°C. A ship date is required. Available to US customers only.
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E.coli RNA Polymerase, Core Enzyme, New England Biolabs
Supplier: New England Biolabs (NEB)
E.coli RNA Polymerase Core Enzyme consists of 5 subunits designated a, a, b´, β, and ω. The enzyme is free of sigma factor and does not initiate specific transcription from bacterial and phage DNA promoters. The enzyme retains the ability to transcribe RNA from nonspecific initiation sequences. Addition of sigma factors will allow the enzyme to initiate RNA synthesis from specific bacterial and phage promoters. The core enzyme has a molecular weight of approximately 400 kDa.
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OneTaq Quick-Load DNA Polymerase, New England Biolabs
Supplier: New England Biolabs (NEB)
OneTaq Quick-Load DNA Polymerase is an optimized, ready-to-use blend of Taq and Deep Vent DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products.
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NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 4), New England Biolabs
Supplier: New England Biolabs (NEB)
For optimal workflow flexibility, NEBNext® Multiplex Oligos are available separately from library preparation kits, providing adaptors and primers for high-yield multiplex Illumina® library production.
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Taq PCR Kit, New England Biolabs
Supplier: New England Biolabs (NEB)
The Taq PCR Kit contains a sufficient supply of recombinant, highly purified Taq DNA Polymerase, PCR-qualified buffer solutions, deoxynucleotides and a broad-range, pre-mixed, ready-to-load DNA marker to perform 200 PCR reactions.
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ONETAQ® QUICK-LOAD® 2X Master Mix with Standard Buffer, New England Biolabs
Supplier: New England Biolabs (NEB)
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep VentR ™ DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products.
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BsaI-HF®v2, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (CutSmart® Buffer).
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OmniTemplate™ for Single Tube Preparation of PCR Template DNA, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' OmniTemplate™ is specifically designed for the rapid isolation of a DNA template for polymerase chain reaction (PCR) analysis from mammalian tissue samples, blood and cell cultures
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Monarch® RNA Cleanup Kits (10 μg), New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® RNA cleanup kit enables fast and simple purification and concentration of up to 10 µg of RNA from enzymatic reactions.
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EPIMARK® Methylated DNA Enrichment Kit, New England Biolabs
Supplier: New England Biolabs (NEB)
The EpiMark Methylated DNA Enrichment Kit enables the enrichment of double-stranded CpG methylated DNA based on CpG methylation density.
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Promega Go Taq Green Master Mix
Supplier: PROMEGA SUNNYVALE INC SE
Pre-mixed, and ready to use, this master mix contains DNA polymerase, dNTPs, MgCl₂, and reaction buffers at optimal concentrations for the amplification of DNA via PCR. Its special formulation allows for direct loading of PCR samples into agarose gels as well as tracking the migration during electrophoresis without the need for loading dyes. Simply add template, primers, and nuclease-free water, available separately. Mater Mix reduces risk of contamination. Includes enough for 100 reactions. Note: Can be stored at 4 °C for up to six weeks, or −20 °C for longer.
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HindIII-HF®, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
HindIII-HF® is an E. coli strain that carries the cloned and modified HindIII gene from Haemophilus influenzae Rd (ATCC 51907). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.
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Pierce™ Immunoprecipitation, Magnetic ChIP Kit, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.
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NEBNEXT® Poly(A) mRNA Magnetic Isolation Module, New England Biolabs
Supplier: New England Biolabs (NEB)
The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA.
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Immobilized T4 DNA Ligase, New England Biolabs
Supplier: New England Biolabs (NEB)
Immobilized T4 DNA Ligase (IM-T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg per ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, enzyme can be removed using a magnet thereby avoiding heat inactivation, and can be re-used.
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SpeI-HF®, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
An E. coli strain that carries the cloned and modified SpeI gene from Sphaerotilus species (ATCC 13923). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
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SpeI-HF®, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
An E. coli strain that carries the cloned and modified SpeI gene from Sphaerotilus species (ATCC 13923). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
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Zymo-Seq™ Cell Free DNA WGBS Library Kit
Supplier: Zymo Research
Prepare robust methyl-seq libraries in as little as three steps.
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Monarch® Spin Collection Tubes
Supplier: New England Biolabs (NEB)
Monarch® Spin collection tubes are designed to be used with Monarch spin columns.
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miRNA Isolation Kit, IBI Scientific
Supplier: IBI Scientific
The miRNA Isolation kits are designed for the purification of microRNA (miRNA) and other small cellular RNAs from tissue samples and cultured cells
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BamHI-HF®, New England Biolabs, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the cloned and modified BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
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Axygen® Mag Plant Genomic DNA Kit, Corning
Supplier: Corning
The AxyPrep Mag Plant genomic DNA kit is a magnetic beads based purification system that allows rapid and reliable isolation of high quality gDNA from multiple plant samples
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E.Z.N.A.® FastFilter Plasmid DNA Mini Kits, Omega Bio-tek
Supplier: Omega Bio-Tek
Isolate plasmid DNA from up to 5 ml culture in 9 minutes utilizing an innovative lysate clearance column.
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Maxi Fast-Ion Plasmid Kit, Endotoxin Free, IBI Scientific
Supplier: IBI Scientific
IBI's Maxi Fast-Ion Plasmid Kits are endotoxin free and use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures
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AccuBlue® and AccuClear® dsDNA Quantitation Kits, Biotium
Supplier: Biotium
AccuBlue™ and AccuClear™ dsDNA quantitation assays provide sensitive, accurate and easy-to-use dsDNA quantitation assays for fluorescent microplate readers in 96-well or 384-well formats.
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BamHI-HF® Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the cloned and modified BamHI gene from Bacillus amyloliquefaciens H (ATCC 49763). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
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ZymoScript RT PreMix Kit
Supplier: Zymo Research
ZymoScript RT PreMix is an optimized reverse transcription kit containing all the necessary components for quick and efficient cDNA synthesis.
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DNA/RNA Shield Fecal Collection Tube (with Beads)
Supplier: Zymo Research
DNA/RNA shield fecal collection tubes are designed for the collection and preservation of nucleic acids from stool specimens.