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Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

TRIS-HEPES-native running buffer (10X), pH 8.0, Thermo Scientific

Supplier: Thermo Scientific Chemicals

TRIS-HEPES-native running buffer (10X), pH 8.0, Thermo Scientific

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TBE (5X), TRIS + borate + EDTA, Thermo Scientific

Supplier: Thermo Scientific Chemicals

TBE (5X), TRIS + borate + EDTA, Thermo Scientific

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Alkaline running buffer solution, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Alkaline running buffer solution, Thermo Scientific

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Laemmli SDS sample buffer, reducing (4X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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PreMixed Running Buffers, IBI Scientific

PreMixed Running Buffers, IBI Scientific

Supplier: IBI Scientific

IBI provides a variety of premixed buffers in various configurations

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Blocking Buffers, Rockland Immunochemicals

Supplier: Rockland Immunochemical

Use BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk

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2 M Tris-Acetate, 0.05 M EDTA (pH8.3)

Supplier: G-Biosciences

2M Tris-Acetate, 0.05M EDTA (pH8.3)

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Rockland Immunochemical

50X Tris-Acetate-EDTA (TAE) (2.0 M Tris-Acetate, 0.05 M EDTA, pH 8.3)

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Laemmli SDS sample buffer, non-reducing (6X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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TNT Buffer Solution, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Pierce™ Blocker™ Casein in PBS, Thermo Scientific

Pierce™ Blocker™ Casein in PBS, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Blocker Casein in PBS or TBS is a ready-to-use, solution of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

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Pierce™ Fast Wash Buffer, 10X, Thermo Scientific

Pierce™ Fast Wash Buffer, 10X, Thermo Scientific

Supplier: Thermo Scientific

Pierce Fast Wash Buffer, 10X, for use with Pierce Fast Blocking Buffer.

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Pierce™ StartingBlock™ (PBS) Blocking Buffer, Thermo Scientific

Pierce™ StartingBlock™ (PBS) Blocking Buffer, Thermo Scientific

Supplier: Thermo Scientific

These StartingBlock™ blocking buffers are PBS and TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and 96-well plate (ELISA) assays.

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IEF Sample buffer (4X) pH 3-7 with lysine, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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MES-SDS running buffer (20X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

MES-SDS running buffer (20X), Thermo Scientific

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Pierce™ Wash Buffers, Thermo Scientific

Pierce™ Wash Buffers, Thermo Scientific

Supplier: Thermo Scientific

These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.

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Pierce™ Blocker™ BLOTTO Blocking Buffer, Thermo Scientific

Pierce™ Blocker™ BLOTTO Blocking Buffer, Thermo Scientific

Supplier: Thermo Scientific

Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other Detection methods.

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SSC buffer (20X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Blue Protein Loading Dye, New England Biolabs

Blue Protein Loading Dye, New England Biolabs

Supplier: New England Biolabs (NEB)

The Blue Loading Buffer Pack contains 3X Blue Loading Buffer (8ml) and 30X Reducing Agent (1ml).

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Laemmli SDS sample buffer, non-reducing (4X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)

Supplier: G-Biosciences

Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.

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G-Biosciences Tris Buffered Saline, 10X

Supplier: G-Biosciences

TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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Citrate-Buffered Saline (20X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Saline-sodium citrate (SSC) buffer.

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Monarch® RNA Cleanup Wash Buffer, New England Biolabs

Monarch® RNA Cleanup Wash Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

This is a component of the Monarch® RNA cleanup kits, is used to wash RNA after binding to ensure the removal of contaminants such as enzyme, NTPs, and short RNA fragments. It is supplied as a 5X concentrate and requires dilution with ethanol (≥95%) before use.

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TrueBlack® WB Blocking Buffer Kit, Biotium

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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SuperBlock™ T20 (TBS) Blocking Buffer, Thermo Scientific

SuperBlock™ T20 (TBS) Blocking Buffer, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific SuperBlock™ Blocking Buffer is optimised solutions in PBS or TBS containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

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