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Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

PBS, Phosphate Buffered Saline Tablets, MilliporeSigma

Supplier: MilliporeSigma

Dissolving one tablet in 1 liter of deionized H₂O yields 140 mM NaCl, 10 mM phosphate buffer, and 3 mM KCl, pH 7.4 at 25°C.

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TE Buffer, Concentrate, OmniPur*. 100X.

Supplier: MilliporeSigma

Colorless, hazefree liquid

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LDS-sample buffer (4X), non-reducing, Thermo Scientific

Supplier: Thermo Scientific Chemicals

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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TAE Buffer, Concentrate, OmniPur*. 25X.

Supplier: MilliporeSigma

Premixed powder

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4X SDS Sample Buffer, MilliporeSigma

Supplier: MilliporeSigma

The 4X SDS Sample Buffer is a standard formulation commonly used for SDS-PAGE analysis of proteins

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SSPE Buffer, 20X, Liquid Concentrate, OmniPur*.

Supplier: MilliporeSigma

Each liter of concentrate makes 20L of 1X SSPE (0.150M NaCl, 0.010M Sodium Phosphate, 0.001M EDTA).

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TBE Buffer, Concentrate, OmniPur®. 10X.

Supplier: MilliporeSigma

Liquid

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SDS-Page Running Gel Buffers, 10X Concentrate

Supplier: Rockland Immunochemical

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature

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SDS-Page Running Gel Buffers, Rockland

Supplier: Rockland Immunochemical

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature

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PreMixed Running Buffers, IBI Scientific

PreMixed Running Buffers, IBI Scientific

Supplier: IBI Scientific

IBI provides a variety of premixed buffers in various configurations

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AccuGENE® PBS (phostphate buffered saline) Buffers, Lonza

AccuGENE® PBS (phostphate buffered saline) Buffers, Lonza

Supplier: Lonza

AccuGENE™ Molecular Biology Buffers are ready-to-use solutions ideal for a wide range of molecular biology applications

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Pierce™ Lane Marker Non-Reducing Sample Buffer, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.

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Pierce™ 1-Step Transfer Buffer, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

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TBE Buffer Solution

Supplier: Biotium

5X TBE for preparation of agarose gel running buffer.

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Blocking Buffer, TBS Fish Gel 10X, Rockland

Supplier: Rockland Immunochemical

Highly sensitive ELISA assays require minimal non-specific interactions

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femtoTBST™ Wash Buffer, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' femto TBST™ will enhance sensitivity of your immunoassays by minimizing the "washing out" of immuno agents or immuno complexes, a common problem associated with classical TBST buffers

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Section Block, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences

Blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates use to charged surface of the slide and tissue section.

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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7.5, Rockland

Supplier: Rockland Immunochemical

Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics

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BLOK™ Casein, 1% Casein Protein Blocking Solution, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' BLOK™ Casein is a purified protein, used for blocking free binding sites in western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

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FirstChoice™ Blocking Agents, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications.

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TRIS-buffered saline (TBS, 10X) pH 7.6, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Wash buffer, 0.5% Tween 20, pH 8.0, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Separating or resolving buffer (4X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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SSPE 20X Hybridisation Buffer

Supplier: Thermo Scientific Chemicals

Liquid

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G-Biosciences Hybridization Buffer (Hybridization Neutralizing Solution)

G-Biosciences Hybridization Buffer (Hybridization Neutralizing Solution)

Supplier: G-Biosciences

Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.

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TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8.3, Thermo Scientific

Supplier: Thermo Scientific Chemicals

For TRIS-Tricine SDS gel electrophoresis

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Nitrocellulose stripping buffer (10X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Running Buffers, Teknova

Supplier: Teknova

Teknova buffers are specifically formulated to comply with current molecular biology protocols

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