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Electrophoresis Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

1X TBS pH 7.8

Supplier: Rockland Immunochemical

1X Tris buffered saline (TBS) pH 7.8

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10X PBS pH 7.2

Supplier: Rockland Immunochemical

10X Phosphate buffered saline (PBS) pH 7.2

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1X PBS Buffer with 1% Casein

Supplier: Rockland Immunochemical

Phosphate buffered saline with casein is suitable for multiple applications including biological diluent buffer for antibodies or other biologics.

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TAE Buffers, Quality Biological

TAE Buffers, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run.

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TBS HCl (10X) Buffer Solution, pH 7.2, Quality Biological

TBS HCl (10X) Buffer Solution, pH 7.2, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Tris HCl, 10X pH 7.2 is a Buffered Saline Solution used in procedures for the isolation of DNA.

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SSPE Buffer, 20X, Rockland Immunochemicals

Supplier: Rockland Immunochemical

Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.

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SSC, 20X Buffer, Quality Biological

SSC, 20X Buffer, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization

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20X SSC Buffer (Standard Sodium Citrate)

Supplier: GROWCELLS

Concentrated sodium chloride-sodium citrate buffer functions by providing high ionic strength to optimize the rate of probe annealing to target nucleic acid

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20X SSC pH 7.0

Supplier: Rockland Immunochemical

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridization, or DNA microarrays).

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Borate Saline Buffered (BSB), Rockland Immunochemicals

Supplier: Rockland Immunochemical

Borate Saline Buffered (BSB) is commonly used in coating procedures for ELISA.

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Blocking Solution, Quality Biological

Blocking Solution, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.

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5X Inlet Buffers

5X Inlet Buffers

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

5x Inlet Buffer, 10mL. Running buffer for use with the Femto Pulse system. 5 concentration.

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DIGE Buffer Kit, Cytiva

DIGE Buffer Kit, Cytiva

Supplier: Cytiva

DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.

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CRITERION™ Phosphate Buffer, pH 7.2

CRITERION™ Phosphate Buffer, pH 7.2

Supplier: HARDY DIAGNOSTICS CA

CRITERION™ Agar, bacteriological grade is used as a solidifying agent in the preparation of solid and semi-solid culture media.

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SSC Buffer, Concentrate, OmniPur*. 20X.

Supplier: MilliporeSigma

Liquid

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TRIS-buffered saline (TBS, 10X) pH 7.4, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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TBE Buffer, Concentrate, OmniPur®. 10X.

Supplier: MilliporeSigma

Liquid

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TAE Buffer, Concentrate, OmniPur*. 25X.

Supplier: MilliporeSigma

Premixed powder

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DNA Gel Loading Buffer, Omega Bio-Tek

Supplier: Omega Bio-Tek

The 50X TAE buffer is composed of 2M tris-acetate and 0.05M EDTA at pH 8.3

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Corning® Tris Base Buffer, Powder, Corning

Corning® Tris Base Buffer, Powder, Corning

Supplier: Corning

Tris is commonly used in buffer solutions in biochemical and molecular biological research

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PBS, Phosphate Buffered Saline Tablets, MilliporeSigma

Supplier: MilliporeSigma

Dissolving one tablet in 1 liter of deionized H₂O yields 140 mM NaCl, 10 mM phosphate buffer, and 3 mM KCl, pH 7.4 at 25°C.

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TE Buffer, Concentrate, OmniPur*. 100X.

Supplier: MilliporeSigma

Colorless, hazefree liquid

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4X SDS Sample Buffer, MilliporeSigma

Supplier: MilliporeSigma

The 4X SDS Sample Buffer is a standard formulation commonly used for SDS-PAGE analysis of proteins

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TAE Buffer, Concentrate, OmniPur*. 10X.

Supplier: MilliporeSigma

Liquid.

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SSPE Buffer, 20X, Liquid Concentrate, OmniPur*.

Supplier: MilliporeSigma

Each liter of concentrate makes 20L of 1X SSPE (0.150M NaCl, 0.010M Sodium Phosphate, 0.001M EDTA).

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MOPS/SDS, 20X, Running Buffer, Teknova

Supplier: Teknova

MOPS/SDS running buffer is designed for protein electrophoresis.

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Electrophoresis Running Buffer

Electrophoresis Running Buffer

Supplier: Edvotek

Tris Acetate, pH 7.8, concentrated 50X (for 5 L—20 standard gels).

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Electrophoresis Running Buffers, G-Biosciences

Supplier: G-Biosciences

G-Biosciences offers many electrophoresis running buffers including: TAE Running Buffer [50X], TBE Running Buffer [10X], TBE-Urea Sample Buffer, MOPS Running Buffer [10X], and MOPS/ SDS Running Buffer [20X]

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PreMixed Running Buffers, IBI Scientific

PreMixed Running Buffers, IBI Scientific

Supplier: IBI Scientific

IBI provides a variety of premixed buffers in various configurations

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Tris-Glycine Buffer

Tris-Glycine Buffer

Supplier: Edvotek

Running buffer for native PAGE.

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