The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
10X PBS pH 7.2
Supplier: Rockland Immunochemical
10X Phosphate buffered saline (PBS) pH 7.2
Expand 1 Items
1X PBS Buffer with 1% Casein
Supplier: Rockland Immunochemical
Phosphate buffered saline with casein is suitable for multiple applications including biological diluent buffer for antibodies or other biologics.
Expand 2 Items
TAE Buffers, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run.
Expand 4 Items
TBS HCl (10X) Buffer Solution, pH 7.2, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Tris HCl, 10X pH 7.2 is a Buffered Saline Solution used in procedures for the isolation of DNA.
Expand 1 Items
SSPE Buffer, 20X, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.
Expand 1 Items
SSC, 20X Buffer, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization
Expand 2 Items
20X SSC Buffer (Standard Sodium Citrate)
Supplier: GROWCELLS
Concentrated sodium chloride-sodium citrate buffer functions by providing high ionic strength to optimize the rate of probe annealing to target nucleic acid
Expand 4 Items
20X SSC pH 7.0
Supplier: Rockland Immunochemical
Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridization, or DNA microarrays).
Expand 1 Items
Borate Saline Buffered (BSB), Rockland Immunochemicals
Supplier: Rockland Immunochemical
Borate Saline Buffered (BSB) is commonly used in coating procedures for ELISA.
Expand 1 Items
Blocking Solution, Quality Biological
Supplier: QUALITY BIOLOGICAL, INC.
Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.
Expand 1 Items
5X Inlet Buffers
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
5x Inlet Buffer, 10mL. Running buffer for use with the Femto Pulse system. 5 concentration.
Expand 2 Items
DIGE Buffer Kit, Cytiva
Supplier: Cytiva
DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.
Expand 1 Items
CRITERION™ Phosphate Buffer, pH 7.2
Supplier: HARDY DIAGNOSTICS CA
CRITERION™ Agar, bacteriological grade is used as a solidifying agent in the preparation of solid and semi-solid culture media.
Expand 2 Items
TRIS-buffered saline (TBS, 10X) pH 7.4, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
Expand 3 Items
DNA Gel Loading Buffer, Omega Bio-Tek
Supplier: Omega Bio-Tek
The 50X TAE buffer is composed of 2M tris-acetate and 0.05M EDTA at pH 8.3
Expand 1 Items
Corning® Tris Base Buffer, Powder, Corning
Supplier: Corning
Tris is commonly used in buffer solutions in biochemical and molecular biological research
Expand 3 Items
PBS, Phosphate Buffered Saline Tablets, MilliporeSigma
Supplier: MilliporeSigma
Dissolving one tablet in 1 liter of deionized H₂O yields 140 mM NaCl, 10 mM phosphate buffer, and 3 mM KCl, pH 7.4 at 25°C.
Expand 1 Items
TE Buffer, Concentrate, OmniPur*. 100X.
Supplier: MilliporeSigma
Colorless, hazefree liquid
Expand 1 Items
4X SDS Sample Buffer, MilliporeSigma
Supplier: MilliporeSigma
The 4X SDS Sample Buffer is a standard formulation commonly used for SDS-PAGE analysis of proteins
Expand 1 Items
SSPE Buffer, 20X, Liquid Concentrate, OmniPur*.
Supplier: MilliporeSigma
Each liter of concentrate makes 20L of 1X SSPE (0.150M NaCl, 0.010M Sodium Phosphate, 0.001M EDTA).
Expand 1 Items
MOPS/SDS, 20X, Running Buffer, Teknova
Supplier: Teknova
MOPS/SDS running buffer is designed for protein electrophoresis.
Expand 1 Items
Electrophoresis Running Buffer
Supplier: Edvotek
Tris Acetate, pH 7.8, concentrated 50X (for 5 L—20 standard gels).
Expand 1 Items
Electrophoresis Running Buffers, G-Biosciences
Supplier: G-Biosciences
G-Biosciences offers many electrophoresis running buffers including: TAE Running Buffer [50X], TBE Running Buffer [10X], TBE-Urea Sample Buffer, MOPS Running Buffer [10X], and MOPS/ SDS Running Buffer [20X]
Expand 20 Items
PreMixed Running Buffers, IBI Scientific
Supplier: IBI Scientific
IBI provides a variety of premixed buffers in various configurations