Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

Supplier: Cytiva

Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Supplier: Cytiva

Capto MMC is a multimodal cation exchanger with a different selectivity, compared with traditional ion exchangers.

Supplier: Cytiva

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

Supplier: Cytiva

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

Supplier: Cytiva

rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.

Supplier: Cytiva

A chromatography resin for purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®. These proteins bind very specifically to the immobilized Strep-Tactin XT ligand giving highly pure target protein.

Supplier: Immunoreagents

ImmunoReagents Donkey IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins

Supplier: Cytiva

Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

Supplier: Cytiva

Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Supplier: Cytiva

GammaBind Plus Sepharose is GammaBind G, Type 3, covalently immobilized to Sepharose CL-6B by malimide linkage.

Supplier: Cytiva

Benzamidine Sepharose 4 Fast Flow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein.

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

Supplier: Cytiva

MabSelect is a protein A affinity resin designed for capturing mAbs from large sample volumes.

Supplier: Cytiva

nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well established Sepharose 4 Fast Flow base matrix.

Supplier: Cytiva

Sephadex G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.

Supplier: Cytiva

SOURCE 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

Supplier: Cytiva

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.

Supplier: Cytiva

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

Supplier: Cytiva

Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.

Supplier: Cytiva

Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades.

Supplier: Cytiva

Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Supplier: MilliporeSigma

Ni-NTA HisBind® Resin is a high-performance Ni²⁺-charged agarose used for rapid one-step purification of proteins containing a HisTag® sequence by metal chelation chromatography.

Supplier: G-Biosciences

G-Biosciences' Nickel Chelating Resin is an immobilized metal affinity chromatography (IMAC) resin that was specifically designed to utilize Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

Supplier: Thermo Scientific

Pierce™ Protein A/G agarose and A/G Plus Agarose is a affinity resin for antibody purification.

Supplier: MilliporeSigma

GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Supplier: MilliporeSigma

Ni-NTA HisBind® Superflow™ Resin is a medium-pressure compatible version of the Ni-NTA HisBind® Resin

Supplier: Thermo Scientific

Thermo Scientific Pierce Protein L Agarose is a premium-quality affinity resin for antibody purification.

Supplier: MilliporeSigma

D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.