You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
SensoLyte® Luminescent Secreted Alkaline Phosphatase Reporter Gene Assay Kit Luminometric
Supplier: Anaspec Inc
The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression including kinetic analysis over time in cell culture or animals owing to its unique ability to secrete into culture medium and serum
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SensoLyte® Generic MMP Assay Kit (Colorimetric), AnaSpec Inc.
Supplier: Anaspec Inc
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components
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SensoLyte® Homogeneous Rh110 Caspase-3/7 Assay Kit
Supplier: Anaspec Inc
Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7
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SensoLyte® 520 Mouse Renin Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec Inc
Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I
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SensoLyte® Thioflavin T β-Amyloid (1-40) Aggregation Kit, AnaSpec
Supplier: Anaspec Inc
The SensoLyte® ThT β-Amyloid (1-40) Aggregation kit provides a convenient and standard method to measure Aβ40 aggregation using Thioflavin T dye
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SensoLyte® Rh110 Matriptase Activity Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec Inc
Matriptase (also known as MT-SP1, ST14, TADG-15 and epithin) is a trypsin-like protease from the family of type II transmembrane serine proteases
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SensoLyte® 520 MMP-9 Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec Inc
MMP-9 (gelatinase-B, collagenase-IV) is involved in quite a few diseases such as a cancer, angiogenesis, alopecia and metastasis.
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Human ADRA1B Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's adrenoreceptor alpha 1B (ADRA1B) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1B.
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Human ADRB1 Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's adrenoreceptor beta 1 (ADRB1) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRB1.
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SensoLyte® Anti-Mouse/ Rat β-Amyloid (1-40) Quantitative ELISA (Colorimetric), AnaSpec
Supplier: Anaspec Inc
This SensoLyte® high-sensitivity (2pg/mL) beta-Amyloid (1-40) Quantitative ELISA Kit (Mouse/Rat) provides a convenient and quantitative assay for determining mouse/rat beta-Amyloid (1-40) (Aβ40) amount in cell and tissue lysate as well as in body fluids
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SensoLyte® Homogeneous AMC Caspase-3/7 Assay Kit, AnaSpec Inc.
Supplier: Anaspec Inc
Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7
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SensoLyte® 440 West Nile Virus Protease Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec Inc
West Nile virus (WNV) causes severe neurological disease and fatalities in both human and animal hosts
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SensoLyte® 490 MMP-8 Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec Inc
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components
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SensoLyte® 520 HCV Protease Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec Inc
The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL® 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format.
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SensoLyte® 520 ADAMTS13 Activity Assay (Fluorimetric), AnaSpec
Supplier: Anaspec Inc
ADAMTS13 (A Disintegrin-like and Metalloprotease with Thrombospondin type-1 motifs 13) is a metalloproteinase that is primarily synthesized in liver cells and secreted into blood circulation
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SensoLyte® 490 MMP-2 Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec Inc
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components
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SensoLyte® 490 MMP-3 Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec Inc
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components
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Human ADRA1A Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's adrenoreceptor alpha 1A (ADRA1A) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1A.
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Human TrkB Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's tropomyosin receptor kinase B (TrkB) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against TrkB.
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SensoLyte® 570 West Nile Virus Protease Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec Inc
West Nile virus (WNV) causes severe neurological disease and fatalities in both human and animal hosts
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Human c-MET/HGFR Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's c-MET/hepatocyte growth factor receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against c-MET (HGFR).
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SensoLyte® Anti-MOG(35-55) IgG Quantitative ELISA Kit (Mouse/Rat), AnaSpec
Supplier: Anaspec Inc
MOG peptide fragment (35-55) induces autoantibody production and relapsing-remitting neurological disease causing extensive plaque-like demyelination
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SensoLyte® Anti-Mouse MOG (1-125) IgG Quantitative ELISA Kit, AnaSpec
Supplier: Anaspec Inc
This kit is optimized to detect anti-mouse MOG (1-125) IgG. Wells are pre-coated with recombinant mouse MOG (1-125) protein and pre-blocked with BSA. The amount of anti-mouse MOG IgG in serum or cerebrospinal fluid is quantified using ELISA. Ample materials and reagents are provided to perform 96 assays.
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SensoLyte® AMC DPPIV Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec Inc
DPPIV is a widely distributed serine protease that cleaves two amino acids from small peptides containing alanine or proline in the second position of the N-terminus of the peptide
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Human IGF-1R Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's insulin-like growth factor receptor 1 reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against IGF-1R.
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Human G-CSFR Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's Granulocyte Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against G-CSFR.
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Human HNF4a Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's hepatocyte nuclear factor 4 alpha (HNF4a) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against HNF4a.
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Human TGR5 Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's G protein-coupled bile acid receptor (TGR5; GPBAR1) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against TGR5 (GPBAR1).
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Anti-Mouse/ Rat β-Amyloid (1-42) Quantitative ELISA (Colorimetric), AnaSpec
Supplier: Anaspec Inc
This SensoLyte® high-sensitivity (2pg/mL) beta-Amyloid (1-42) Quantitative ELISA Kit (Mouse/Rat) provides a convenient and quantitative assay for determining mouse/rat beta-Amyloid (1-42) (Aβ42) amount in cell and tissue lysate as well as in body fluids
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SensoLyte® Plus 520 MMP-9 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.
Supplier: Anaspec Inc
The SensoLyte® Plus 520 MMP-9 Assay Kit is designed for specifically detecting MMP-9 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-9 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL®520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-9-induced cleavage of the FRET substrate. Ample materials are provided to perform 96 assays in a 96-well format.