You searched for: Enzyme Assays

Supplier: Anaspec Inc

Alkaline phosphatase is a popular enzyme conjugated with secondary antibody for ELISA

Supplier: Anaspec Inc

The SensoLyte® Rh110 Elastase Assay Kit detects elastase activity using a fluorogenic peptide substrate. Upon elastase cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496 /520 nm. The increase in fluorescence intensity is directly proportional to enzyme activity. The kit does not require any separation steps and can be used to continuously measure the kinetics of elastase. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from screening compounds.

Supplier: Anaspec Inc

GST (Glutathione S-Transferase) enzymes, a family of isozymes plays a crucial role in body defense mechanisms against toxins and carcinogens

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Peptidyl-prolyl isomerase (PPIase) Pin1 catalyzes cis/trans isomerization of the phospho-Serine/Threonine-Proline peptide bonds

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Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators.

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Enterokinase is a heterodimeric serine protease produced by cells in the duodenal wall

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Peroxidases catalyze oxidation-reduction reactions and play an important role in protecting cell from oxidative injury

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Glutathione (GSH) is an important antioxidant molecule that functions as a cofactor for a variety of enzymes and is involved in various biological processes

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Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins

Supplier: INDIGO BIOSCIENCES INC MS

Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's fibroblast growth factor receptor 1c and α-klotho reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against FGFR1/α-klotho.

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Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I

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The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression including kinetic analysis over time in cell culture or animals owing to its unique ability to secrete into culture medium and serum

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Nicotinamide nucleotides are key players in the energy transforming and oxidation-reduction reactions of a cell

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Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins

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Matriptase (also known as MT-SP1, ST14, TADG-15 and epithin) is a trypsin-like protease from the family of type II transmembrane serine proteases

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Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme)

Supplier: INDIGO BIOSCIENCES INC MS

Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's adrenoreceptor alpha 1D (ADRA1D) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1D.

Supplier: INDIGO BIOSCIENCES INC MS

Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. Macrophage colony-stimulating factor receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against M-CSFR.

Supplier: INDIGO BIOSCIENCES INC MS

Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's tropomyosin receptor kinase A reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against TrkA (NTRK1).

Supplier: Anaspec Inc

Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme)

Supplier: Anaspec Inc

Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins

Supplier: Anaspec Inc

Deubiquitinating enzymes (DUBs) are proteases that reverse ubiquitin modifications and can rescue a protein from proteasomal degradation

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Plasmin belongs to the family of serine proteases

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West Nile virus (WNV) causes severe neurological disease and fatalities in both human and animal hosts

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Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity

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The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL® 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format.

Supplier: Anaspec Inc

Aggrecanases belong to ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases

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Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7

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Cathepsin E is a non-lysosomal aspartic proteinase of the pepsin superfamily, playing an important role in host defense against cancer cells in addition to it’s roles in protein degradation, antigen processing etc

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Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation