You searched for: Enzyme Assays

Supplier: Enzo Life Sciences

A FLUOR DE LYS® fluorescent assay system

Supplier: Enzo Life Sciences

The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases

Supplier: Enzo Life Sciences

An improved Fluor de Lys® HDAC assay with Fluor de Lys®-Green, a new substrate offering higher sensitivity and an excitation and emission (485/530nm) that avoids quenching and fluorescent interference from compounds absorbing in the near UV and blue range.The Fluor de Lys®-Green HDAC assay is a complete kit for measuring histone deacetylase (HDAC) activity in cell or nuclear extracts, immunoprecipitates or purified enzymes

Supplier: Enzo Life Sciences

The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay.

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay SystemThe MMP-7 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-7 (MMP-7, matrilysin) using a thiopeptide as a chromogenic substrate.

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: Enzo Life Sciences

For the quantitative determination of PKA kinase activity in partially purified, purified, or crude enzyme preparations from any species.

Supplier: G-Biosciences

G-Biosciences' β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct.

Supplier: Enzo Life Sciences

For the measurement of PKC kinase activity partially purified, purified, or crude enzyme preparations from any species. Cited sample type includes tissue (ref. 1).

Supplier: G-Biosciences

Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation

Supplier: MOLECULAR DEVICES LLC. CA

IMAP® technology provides a homogeneous assay applicable to a wide variety of kinases without regard for substrate peptide sequence.

Supplier: Enzo Life Sciences

The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.

Supplier: G-Biosciences

The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample

Supplier: G-Biosciences

G-Biosciences' Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct.

Supplier: Enzo Life Sciences

The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit.

Supplier: Enzo Life Sciences

The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, a potential therapeutic target, providing enough material to perform at least 96 assays.

Supplier: Enzo Life Sciences

For the measurement of glutathione peroxidase in cell lysates, plasma, and tissue from any species.

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay SystemThe MMP-9 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-9 (MMP-9, gelatinase B) using a quenched fluorogenic peptide: OmniMMPTM fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin K LeuArg peptide recognition sequence, this kit allows the detection of cathepsin K activity in living cells by fluorescence microscopy.

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy.

Supplier: Enzo Life Sciences

The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.

Supplier: Enzo Life Sciences

The AEC Peroxidase Substrate Kit contains reagents for visualization of peroxidase reactions using Aminoethylcarbazole (AEC). In the presence of H2O2, peroxidases catalyze the oxidation of AEC into a brick red precipitate. The supplied reagents are sufficient for preparation of 300 ml of ready-to-use color reaction mixture.

Supplier: Enzo Life Sciences

BIOMOL Green™ provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL Green™ doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL Green™ is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL Green™ has perhaps been most widely applied to protein phosphatase assays. Typically, a protein phosphatase (e.g. PTP1B or LAR) is simply incubated with an appropriate phosphopeptide (e.g. IR5,9,10). Addition of BIOMOL Green™ stops the reaction and begins color development, which is read 20-30 min. later.BIOMOL Green™ may be used in cuvette or microplate-based assays and is ideal for high-throughput applications. Each kit comes with a standardized phosphate solution for assay calibration.

Supplier: Enzo Life Sciences

It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.

Supplier: BIOASSAY SYSTEMS

For quantitative determination of fumarase enzyme activity.

Supplier: BIOASSAY SYSTEMS

For quantitative determination of phosphoglucomutase enzyme activity in biological samples.

Supplier: BIOASSAY SYSTEMS

For quantitative determination of FTase enzyme activity in biological samples.

Supplier: BIOASSAY SYSTEMS

For quantitative determination of alpha-mannosidase enzyme activity.