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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

ToxiLight® Non-Destructive Cytotoxicity BioAssay Kit

ToxiLight® Non-Destructive Cytotoxicity BioAssay Kit

Supplier: LONZA PHARMA - BIOSCIENCE CA

The ToxiLight® BioAssay Kit is a bioluminescent, non-destructive cytolysis assay kit designed to measure the release of the enzyme, adenylate kinase (AK), from damaged cells. AK is a robust protein present in all eukaryotic cells, which is released into the culture medium when cells die.

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Bacterial Gram Stains and Viability Kits, Biotium

Supplier: Biotium

Kits to detect live and dead bacteria, and perform Gram stain using fluorescent wheat germ agglutinin (WGA) conjugate.

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Dengue Virus Reporter Virus Particle (RVP) Renilla Luciferase

Dengue Virus Reporter Virus Particle (RVP) Renilla Luciferase

Supplier: ReVacc Scientific

Reporter virus particles (RVPs) package the capsid-preMembrane-envelope (C-prM-E) gene construct of Dengue virus (DENV) (GenBank: MK506263.1, Serotype 2 strain) and a Renilla luciferase reporter.

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Apoptosis and Necrosis Quantitation Kits, Biotium

Apoptosis and Necrosis Quantitation Kits, Biotium

Supplier: Biotium

Biotium's apoptosis/necrosis quantitation kits pair green fluorescent FITC-Annexin V conjugate with the membrane impermeant red fluorescent nucleic acid stain ethidium homodimer III

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Apoptosis Inducers, G-Biosciences

Apoptosis Inducers, G-Biosciences

Supplier: G-Biosciences

Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

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Amplite® Fluorimetric Lithium Ion Quantification Kit

Amplite® Fluorimetric Lithium Ion Quantification Kit

Supplier: AAT BIOQUEST INC

Designed for consistent and dependable results. Amplite® fluorimetric lithium ion quantification kit uses our new robust lithium-ion indicator dye, Lithiumighty™ 520, which exhibits great fluorescence intensity enhancement upon binding to lithium ions.

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SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit, New England Biolabs

SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit, New England Biolabs

Supplier: New England Biolabs (NEB)

The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes isothermal amplification for use in the analysis of SARS-CoV-2, the novel coronavirus that causes COVID-19.

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Tempo-iKupffer™ Human iPSC-derived Kupffer Cells

Supplier: TEMPO BIOSCIENCE, INC

Kupffer cells are the resident macrophages in the human liver. Like Tempo-iPhago™ (macrophages are white blood cells that capture and kill microorganisms), Kupffer cells display innate immune functions protecting the liver from bacterial infections and serve as gatekeepers.

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Live-or-Dye NucFix™ Red Staining Kit, Biotium

Supplier: Biotium

Live-or-Dye NucFix™ Red is a red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected by flow cytometry or fluorescence microscopy.

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Live-or-Dye™ Fixable Viability Staining Kits, Biotium

Live-or-Dye™ Fixable Viability Staining Kits, Biotium

Supplier: Biotium

Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.

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JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

Supplier: Biotium

JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.

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ViaVac™ Red/Green (FUN®1), Biotium

ViaVac™ Red/Green (FUN®1), Biotium

Supplier: Biotium

A fluorescent vitality dye for yeast, identical in structure to the dye FUN® 1. Living yeast cells show red fluorescent vacuolar tubules.

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Nitric Oxide (total) Assay Kit, Multispecies, Thermo Scientific

Supplier: Thermo Scientific

Assay nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Nitric Oxide Assay Kit.

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Neutrophil/Monocyte Respiratory Burst Assay Kit, Cayman Chemical Company

Neutrophil/Monocyte Respiratory Burst Assay Kit, Cayman Chemical Company

Supplier: Cayman Chemical Company

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

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NucView® Caspase-3 Enzyme Substrates, Biotium

NucView® Caspase-3 Enzyme Substrates, Biotium

Supplier: Biotium

NucView® Caspase-3 Substrates are novel fluorescent probes that allow real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

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Amplite® Fluorimetric Sodium Ion Quantification Kit

Amplite® Fluorimetric Sodium Ion Quantification Kit

Supplier: AAT BIOQUEST INC

Designed for consistent and dependable results. Amplite® fluorimetric sodium ion quantification kit uses our robust sodium ion indicator dye, SoNa™ 520, which exhibit great fluorescence intensity enhancement upon binding to sodium ions.

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ToxiLight® Non-Destructive Cytotoxicity BioAssay Kit with Plates

ToxiLight® Non-Destructive Cytotoxicity BioAssay Kit with Plates

Supplier: LONZA PHARMA - BIOSCIENCE CA

The ToxiLight® BioAssay Kit is a bioluminescent, non-destructive cytolysis assay kit designed to measure the release of the enzyme, adenylate kinase (AK), from damaged cells. AK is a robust protein present in all eukaryotic cells, which is released into the culture medium when cells die.

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Pierce™ Peroxidase Suppressor, Thermo Scientific

Pierce™ Peroxidase Suppressor, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity from cells and tissues to reduce background signal in HRP-based immunohistochemistry applications.

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Tempo-iHep3D™ Human iPSC-derived Hepatocytes

Supplier: TEMPO BIOSCIENCE, INC

One of the most widely used cell types in research, drug development, and toxicity testing is hepatocytes (liver cells). Existing liver models (for cell culture based systems) include primary human hepatocytes harvested from donors, immortalized cell lines (such as HepG2), and cells from rodent models. Each of these models suffers experimental limitations.

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Tempo-iLSEC™ Human iPSC-derived Liver Sinusoidal Endothelial Cells

Supplier: TEMPO BIOSCIENCE, INC

Liver sinusoidal endothelial cells (LSECs) are the most abundant non-parenchymal hepatic cell population. They are highly specialized liver endothelial cells that form a barrier to separate hepatocytes and hepatic stellate cells from contacting the blood. In additional to serving as a physical barrier, LSECs have critical physiological and immunological functions. Furthermore, LSECs regulate cell-to-cell crosstalk as chronic liver conditions advance, eventually leading to fibrosis and carcinogenesis.

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CF® Dye Dextran 10000 MW, Anionic and Fixable, Biotium

CF® Dye Dextran 10000 MW, Anionic and Fixable, Biotium

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10,000 MW, and contains a formaldehyde-fixable free-amine group.

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CellBrite™ Fix Membrane Stains, Biotium

Supplier: Biotium

CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in which they can withstand both fixation and detergent permeabilisation.

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Tempo-iHepStellate™ Human iPSC-derived Hepatic Stellate Cells

Supplier: TEMPO BIOSCIENCE, INC

Hepatic stellate cells are responsible for the regulation of extracellular matrix proteins and vascular structure in the liver. They represent approximately 5 to 8% of the cells in the liver and function to maintain homeostasis in the microenvironment of the hepatic sinusoid. They play a critical role in the development of liver fibrosis. When chronic liver damage occurs, hepatic stellate cells are actively synthesizing extracellular matrix proteins such as collagen and other glycoproteins. The accumulation of the extracellular matrix proteins trigger hepatic fibrosis which can lead to cirrhosis.

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Nitric Oxide Assay Kit, Multispecies, Thermo Scientific

Supplier: Thermo Scientific

Assay nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Nitric Oxide Assay Kit.Features of the Nitric Oxide Assay Kit:Target: nitric oxide (NO) and relative levels of nitrite (NO2-) and nitrate (NO3-)Format: colorimetric stain (azo dye) for clear 96-well platesMeasurement: absorbance at 540nm (±20nm)Assay Range: 0 to 100µMSensitivity: 0.222µM nitrite; 0.625µM nitratePrecision: dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)Sample Types: urine, saliva, plasma, serum and other biological fluidsTotal Assay Time: 15 minutes (nitrite assay); 45 minutes (nitrate assay)Sample Size: 50 µLThis nitric oxide assay kit is for quantitative determination of nitrite (NO2-) and nitrate (NO3-) in biological fluids

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Annexin V Apoptosis Detection Kit with 7-AAD, PE

Annexin V Apoptosis Detection Kit with 7-AAD, PE

Supplier: STEMCELL Technologies

Apoptosis staining and detection kit - 100 tests.

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ExoQuick Plasma Precipitation Kit, System Biosciences

Supplier: SBI

A single kit optimizes exosome isolation from plasma by combining thrombin for de-fibrination with ExoQuick.

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Human PDGFR a/b Reporter Assay System, INDIGO Biosciences

Human PDGFR a/b Reporter Assay System, INDIGO Biosciences

Supplier: INDIGO BIOSCIENCES INC MS

All-inclusive cell-based luciferase reporter assay kit targeting the Platelet-Derived Growth Factor Receptor alpha and beta, key receptors in atherosclerosis, pulmonary fibrosis, angiogenesis, and tumorigenesis research.

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Human PXR Luciferase Reporter Assay Kit

Human PXR Luciferase Reporter Assay Kit

Supplier: INDIGO BIOSCIENCES INC MS

Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Pregnane X Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against PXR (NR1I2).

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ArciTect™ Human CRISPR Optimization Kits (APC, PE, FITC)

ArciTect™ Human CRISPR Optimization Kits (APC, PE, FITC)

Supplier: STEMCELL Technologies

Complete kit for optimization of genome editing in human cells.

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Human AhR Luciferase Reporter Assay Kit

Human AhR Luciferase Reporter Assay Kit

Supplier: INDIGO BIOSCIENCES INC MS

Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Aryl Hydrocarbon Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against AhR.

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