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280 results for Protein Linking and Labeling Systems

You searched for: Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Pierce™ Immunostain Enhancer, Thermo Scientific

Pierce™ Immunostain Enhancer, Thermo Scientific

Supplier: Thermo Scientific

The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.

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Amine Reactive Biotinylation Kits, EZ-Link™, Thermo Scientific

Amine Reactive Biotinylation Kits, EZ-Link™, Thermo Scientific

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

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IMPACT™ Kit for Protein Purification, New England Biolabs

IMPACT™ Kit for Protein Purification, New England Biolabs

Supplier: New England Biolabs (NEB)

The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.

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Chitin Resin, New England Biolabs

Chitin Resin, New England Biolabs

Supplier: New England Biolabs (NEB)

An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion.

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Pierce™ EZ-Link™ Amine Reactive Biotinylation Reagents, Thermo Scientific

Pierce™ EZ-Link™ Amine Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

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CF®DI Aminooxy, Biotium

Supplier: Biotium

CF®DI aminooxy dyes react with aldehyde/ketone groups on proteins or other biomolecules to form a stable oxime bond. The CF®DI dyes (CF®488DI, CF®555DI and CF®647DI) are designed to match each other for molecular weight and charge. The molecular weights of the same protein conjugated to different CF®DI dye colors are close to each other and the pl of the labeled proteins remains the same.

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BG-Maleimide, New England Biolabs

BG-Maleimide, New England Biolabs

Supplier: New England Biolabs (NEB)

BG-Maleimide is a thiol-reactive building block for the one-step synthesis of SNAP-tag® substrates from thiol-containing precursors such as thiol-modified oligonucleotides

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Pierce™ DyLight™ Antibody Labeling, NHS Ester Quenchers, Thermo Scientific

Pierce™ DyLight™ Antibody Labeling, NHS Ester Quenchers, Thermo Scientific

Supplier: Thermo Scientific

DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes

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Pierce™ NHS-Azide and NHS-Phosphine Reagents, Thermo Scientific

Pierce™ NHS-Azide and NHS-Phosphine Reagents, Thermo Scientific

Supplier: Thermo Scientific

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

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Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific

Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific

Supplier: Thermo Scientific

DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.

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Pierce™ PEGylated SPDP Crosslinkers, Thermo Scientific

Pierce™ PEGylated SPDP Crosslinkers, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce™ PEGn-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a n-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

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Pierce™ Pegylation Reagents, Methyl-PEG-Amine Compounds, Thermo Scientific

Pierce™ Pegylation Reagents, Methyl-PEG-Amine Compounds, Thermo Scientific

Supplier: Thermo Scientific

The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers

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Template Switching RT Enzyme Mix, New England Biolabs

Template Switching RT Enzyme Mix, New England Biolabs

Supplier: New England Biolabs (NEB)

The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template.

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Pierce™  ABC Peroxidase Standard Staining Kit, Thermo Scientific

Pierce™ ABC Peroxidase Standard Staining Kit, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

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Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers, Thermo Scientific

Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers, Thermo Scientific

Supplier: Thermo Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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GFP-Traps, Bulldog Bio

GFP-Traps, Bulldog Bio

Supplier: Bulldog Bio

Novel Antibody Fragment that binds GFP Fusion Proteins.

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GloCell™ Fixable Viability Dye Violet 510

GloCell™ Fixable Viability Dye Violet 510

Supplier: STEMCELL Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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GloCell™ Fixable Viability Dye Red 710

GloCell™ Fixable Viability Dye Red 710

Supplier: STEMCELL Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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AnaTag™ B-PE Labeling Kit, Anaspec Inc.

Supplier: Anaspec Inc

The AnaTag™ B-PE Labeling Kit is optimized for use in the conjugation of B-Phycoerythrin (B-PE) to antibodies. B-PE, a fluorescent protein from phycobiliprotein family, has a primary absorption peak at 545 nm with a secondary peak at 563 nm and maximum emission at 578 nm.

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StabilZyme® Protein-Free Stabilizer, Surmodics

Supplier: Surmodics

StabilZyme® HRP, StabilZyme® Protein-Free and StabilZyme® NOBLE stabilizers provide the assay developer a complete set of tools to stabilize different HRP conjugates for long term, in-solution stability.

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AnaTag™ HiLyte™ Fluor 488 Protein Labeling Kit [3 labeling reactions (3×5 mg protein)], Anaspec

Supplier: Anaspec Inc

HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein.

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StabilZyme® Noble Stabilizer, Surmodics

Supplier: Surmodics

StabilZyme® HRP, StabilZyme® Protein-Free and StabilZyme® NOBLE stabilizers provide the assay developer a complete set of tools to stabilize different HRP conjugates for long term, in-solution stability.

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GloCell™ Fixable Viability Dye Violet 540

GloCell™ Fixable Viability Dye Violet 540

Supplier: STEMCELL Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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AnaTag™ HiLyte™ Fluor 555 Protein Labeling Kit *Ultra Convenient* [3 labeling reactions (3 x 5 mg protein)], Anaspec

Supplier: Anaspec Inc

HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy™ 3 dyes, resulting in an optimal match to filters designed for CyTM dyes.

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GloCell™ Fixable Viability Dye Violet 450

GloCell™ Fixable Viability Dye Violet 450

Supplier: STEMCELL Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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AnaTag™ AMCA - X Protein Labeling Kit *Ultra Convenient*, Anaspec

Supplier: Anaspec Inc

AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group.

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GloCell™ Fixable Viability Dye Red 780

GloCell™ Fixable Viability Dye Red 780

Supplier: STEMCELL Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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GloCell™ Fixable Viability Dye UV 450

GloCell™ Fixable Viability Dye UV 450

Supplier: STEMCELL Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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StabilZyme® AP Conjugate stabilizer, Surmodics

Supplier: Surmodics

StabilZyme® AP and StabilZyme® Protein-Free AP stabilizers provide assay developers a complete set of tools to stabilize different AP conjugates for long term, in-solution stability.

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AnaTag™ HiLyte™ Fluor 488 Microscale Protein Labeling Kit, Anaspec

Supplier: Anaspec Inc

HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein.

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