Protein Linking and Labeling Systems

Supplier: Abcam

Labeling in < 20 mins with 30 secs hands-on time using Atto 633 Conjugation Kit (Fast) - Lightning-Link® ab269898. 100% antibody recovery.

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Abcam

20nm Gold conjugation / labeling in < 4 hrs with 30 secs hands-on time using Gold-Maleimide Conjugation Kit (20nm, 20 OD) ab269902. 100% antibody recovery.

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using PE/Atto 594 Conjugation Kit - Lightning-Link® ab269900. 100% antibody recovery.

Supplier: Abcam

iFluor® Red 750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using iFluor® Red 750 Conjugation Kit (BSA compatible) ab312812. 100% antibody recovery.

Supplier: Abcam

Alkaline Phosphatase conjugation / labeling in < 4 hrs with 30 secs hands-on time using Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link® ab269901. 100% antibody recovery.

Supplier: Abcam

Labeling in < 4 hrs with 30 secs hands-on time using 167Er Metal Conjugation Kit - Lightning-Link® ab269846. 100% antibody recovery.

Supplier: Abcam

Abcam's Doublestain IHC Kit (ab183285) is designed for use with user supplied rabbit and rat primary antibodies to detect two distinct antigens on human and mouse tissue or cell samples.

Supplier: Abcam

Alkaline Phosphatase conjugation / labeling in < 4 hrs with 30 secs hands-on time using Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link® ab269901. 100% antibody recovery.

Supplier: Abcam

XFD750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using XFD750 Conjugation Kit (BSA compatible) ab312813. 100% antibody recovery.

Supplier: Enzo Life Sciences

Cell viability dye.

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

Supplier: Abcam

Labeling in < 20 mins with 30 secs hands-on time using DyLight® 405 Conjugation Kit (Fast) - Lightning-Link® ab201798. 100% antibody recovery.

Supplier: Thermo Scientific

Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.

Supplier: Cytiva

Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labeling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

Supplier: Enzo Life Sciences

Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labelling.