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Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Pierce™  ABC Peroxidase Standard Staining Kit, Thermo Scientific

Pierce™ ABC Peroxidase Standard Staining Kit, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.

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Pierce™ Immunostain Enhancer, Thermo Scientific

Pierce™ Immunostain Enhancer, Thermo Scientific

Supplier: Thermo Scientific

The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.

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Pierce™ Magnetic RNA-Protein Pull-Down Kit, Thermo Scientific

Pierce™ Magnetic RNA-Protein Pull-Down Kit, Thermo Scientific

Supplier: Thermo Scientific

The Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit provides researchers with a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait.

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Pierce™ Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Thermo Scientific

Pierce™ Sulfo-SBED Biotin Label Transfer Kit and Reagents - Western Blot Application, Thermo Scientific

Supplier: Thermo Scientific

Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

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Gold-Maleimide Conjugation Kit (40nm, 20 OD), Abcam

Supplier: Abcam

40nm Gold conjugation / labeling in < 4 hrs with 30 secs hands-on time using Gold-Maleimide Conjugation Kit (40nm, 20 OD) ab188290. 100% antibody recovery.

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Phos-tag™ Mass Analytical Kit

Supplier: FUJIFILM BIOSCIENCES INC

Phos-tag™ Mass Analytical Kit is mixed with samples for MALDI-TOF/Mass analysis.

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XFD750 Conjugation Kit, Abcam

Supplier: Abcam

XFD750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using XFD750 Conjugation Kit ab312814. 100% antibody recovery.

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Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific

Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Thermo Scientific

Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

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Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific

Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific

Supplier: Thermo Scientific

The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers

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Pierce™ PEGylated BM (bismaleimido) Crosslinkers, Thermo Scientific

Pierce™ PEGylated BM (bismaleimido) Crosslinkers, Thermo Scientific

Supplier: Thermo Scientific

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions

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Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific

Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific

Supplier: Thermo Scientific

DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.

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Pierce™ GlycoLink™ Coupling Catalyst, Thermo Scientific

Pierce™ GlycoLink™ Coupling Catalyst, Thermo Scientific

Supplier: Thermo Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

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ORGANELLE-ID-RGB® Reagent II, Enzo Life Sciences

ORGANELLE-ID-RGB® Reagent II, Enzo Life Sciences

Supplier: Enzo Life Sciences

Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids.

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Phosphotyrosine detection set, Enzo Life Sciences

Supplier: Enzo Life Sciences

This kit offers a set of seven different MAbs specific for tyrosine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries.

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Aqua 431 dUTP, Enzo Life Sciences

Aqua 431 dUTP, Enzo Life Sciences

Supplier: Enzo Life Sciences

Aqua 431 [DEAC] dUTP is a coumarin derivative that can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

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Rhodamine Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: Abcam

Rhodamine conjugation / labeling in < 20 mins with 30 secs hands-on time using Rhodamine Conjugation Kit (Fast) - Lightning-Link® ab188286. 100% antibody recovery.

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