Protein Extraction

Supplier: Enzo Life Sciences

FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates.

Supplier: CHI Scientific

OptiTDS™ is a proprietary Tissue Dissociation system which enzymatically digests extracellular connective fibers without harming the cells themselves

Supplier: Thermo Scientific

Pierce™ Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss.

Supplier: Abcam

Protein Extraction Kit (ab270054) is for the solubilization of proteins for a variety of proteomics applications.

Supplier: Abcam

5 cells or 1 mg of tissue, with optimal results from >10^6 cells or >10 mg of tissue - High yield – up to 0.4 mg of histone protein per 10^7 cells or 100 mg of tissue - Useful in western blot analysis of histone modifications and post-translational modifications - Not for ChIP; use a chromatin extraction kit for ChIP preparation

Supplier: Thermo Scientific

The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.

Supplier: Abcam

Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).

Supplier: Abcam

Plasma membrane protein extraction kit provides optimized buffers and reagents for the effective extraction of plasma membrane proteins from mammalian tissues and cells. The kit offers the option to either extract total cellular membrane proteins or specifically purify plasma membrane proteins. - Cited in over 200 publications - High yield and purity – consistent yield with high purity (over 90%) - Suitable for western blotting, 2-D gels, enzyme analyses, and more

Supplier: Thermo Scientific

The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.

Supplier: Thermo Scientific

Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.

Supplier: Thermo Scientific

Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications. Store at -20˚C in a nonfrost-free freezer.

Supplier: Thermo Scientific

The Pierce™ Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

Supplier: Abcam

ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues.

Supplier: Abcam

Lipid Extraction Kit (Chloroform-Free) provides a safer, chloroform-free method for efficient lipid isolation from plasma, serum, tissue, and cells. The recovered organic phase can be dried and resuspended for downstream lipid analysis. - Cited in over 15 publications - Supports multiple sample types including - plasma, serum, tissue, suspension cells, and adherent cells. - Chloroform-free - eliminates the use of toxic chloroform in lipid extraction - Efficient lipid isolation – high-yield lipid extraction with minimal contamination - Sufficient for 50 lipid preparations (25 µL sample size)

Supplier: Thermo Scientific

Glu-C Protease, MS Grade, is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications, in-solution digestion of proteins and in-gel digestion of proteins. Store at -20 ˚C in a nonfrost-free freezer.

Supplier: Thermo Scientific

Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.