Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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20x TBS-T with Tween 20, Abcam

Supplier: Abcam

Tris Buffered Saline (TBS) wash buffer with Tween 20 for use during immunolabeling techniques such as IHC.

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PBS Buffered Saline 1X w/o Ca & Mg, Cytiva

Supplier: Cytiva

Cytiva manufactures PBS utilizing USP raw materials formulated in USP Water for Injection quality water.

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Sheath Fluid (30X), Tonbo Biosciences

Supplier: Tonbo Biosciences

Tonbo Biosciences Sheath Fluid (30X) is a pre-filtered, pH balanced phosphate buffered saline solution that can be used for transporting particles though any flow cytometer.

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Neutralizing Reagent, (5 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Neutralizing buffer for low pH solutions, for use with cyclic nucleotide and isoprostane kits.

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TNE Buffer, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

TNE Buffer is often used as a base material to generate lysis buffers.

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Tween-20 solution 10%, Enzo Life Sciences

Supplier: Enzo Life Sciences

This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.

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Pierce™ 1-Step Transfer Buffer, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.