Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Nuclear Extraction Kit, Abcam

Supplier: Abcam

Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation

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HEPES Buffer (1 M), pH 7.3, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent at most biological pH’s and is categorized as an all- purpose buffer available for biological research.

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TNE Buffer, Quality Biological

Supplier: QUALITY BIOLOGICAL, INC.

TNE Buffer is often used as a base material to generate lysis buffers.

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RNA Binding Buffer, Zymo Research

Supplier: Zymo Research

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Viral Wash Buffer (Concentrate), Zymo Research

Supplier: Zymo Research

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Tween-20 solution 10%, Enzo Life Sciences

Supplier: Enzo Life Sciences

This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.

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Kinase Dilution Buffers V

Supplier: Sino Biological

Solution of buffer used for diluting kinase protein.

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Acetyltransferase Dilution Buffers

Supplier: Sino Biological

Solution of buffer used for diluting enzyme histone acetyltransferases (KAT).

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Sheath Fluid (30X), Tonbo Biosciences

Supplier: Tonbo Biosciences

Tonbo Biosciences Sheath Fluid (30X) is a pre-filtered, pH balanced phosphate buffered saline solution that can be used for transporting particles though any flow cytometer.