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Buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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SSPE Buffer, 20X concentrate, pH 7.4, Rockland Immunochemicals

Supplier: Rockland Immunochemical

20X SSPE buffer (3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA, pH 7.4) - DEPC treated.

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BLOT-QuickBlocker™ Modified Milk Protein Blocking Agent, G-Biosciences

Supplier: G-Biosciences

G-Biosciences' BLOT-QuickBlocker™ is a novel modified milk protein that is highly soluble and does not inhibit peroxidase detection

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NEBuffer 2 , New England Biolabs

NEBuffer 2 , New England Biolabs

Supplier: New England Biolabs (NEB)

New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity

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Buffer Assortment Pack, Hydrion Buffer Chemvelopes, Micro Essential Lab

Supplier: Micro Essential

Buffer assortment pack includes buffers with pH ranges from 2 to 11. Chemvelopes are made of a triple protective foil consisting of aluminum, polyethylene and paper which keeps the Hydrion Buffers dry and free-flowing.

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Tris HCl pH 9.0, 1.0 M

Supplier: Rockland Immunochemical

Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology.

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TBS pH 7.5, 10X, Rockland Immunochemicals

Supplier: Rockland Immunochemical

10X Tris buffered saline (TBS) pH 7.5

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PBS (Phosphate Buffered Saline), 1X, with EDTA, pH 7.4, Teknova

Supplier: Teknova

PBS buffer contains 137 mM sodium chloride, 7 mM sodium phosphate dibasic, 3 mM potassium chloride, 1.4 mM potassium phosphate monobasic, 4 mM EDTA pH 7.4

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pDNA Resuspension Buffer, pH 8.0

Supplier: Teknova

pDNA Resuspension buffer composition: 50 mM Tris/Tris-HCl, 10 mM EDTA.

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1X PBS - TBN Buffer, Sterile, Teknova

Supplier: Teknova

1X PBS-TBN Buffer

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Magnesium Sulfate (MgSO₄) Solution, New England Biolabs

Magnesium Sulfate (MgSO₄) Solution, New England Biolabs

Supplier: New England Biolabs (NEB)

100 mM MgSO₄ for use in conjunction with polymerase reaction buffers.

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Monarch® RNA Cleanup Wash Buffer, New England Biolabs

Monarch® RNA Cleanup Wash Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

This is a component of the Monarch® RNA cleanup kits, is used to wash RNA after binding to ensure the removal of contaminants such as enzyme, NTPs, and short RNA fragments. It is supplied as a 5X concentrate and requires dilution with ethanol (≥95%) before use.

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Monarch® gDNA Elution Buffer, New England Biolabs

Supplier: New England Biolabs (NEB)

The Monarch® gDNA Elution Buffer a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify up to 30 µg genomic DNA  from a variety of sample types.

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Acetate Buffer, pH 6.0, EP Buffer Solution 4002200, Ricca Chemical Company

Acetate Buffer, pH 6.0, EP Buffer Solution 4002200, Ricca Chemical Company

Supplier: Ricca Chemical

Suitable for use as Acetate Buffer, pH 6.0 R, per EP Method 4402200

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RIPA Lysis Buffer, Rockland

Supplier: Rockland Immunochemical

RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells

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TrueBlack® WB Blocking Buffer Kit, Biotium

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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Phosphate Buffer, pH 7, for Amperometric Determination of Residual Chlorine and Chlorine Dioxide, Ricca Chemical Company

Phosphate Buffer, pH 7, for Amperometric Determination of Residual Chlorine and Chlorine Dioxide, Ricca Chemical Company

Supplier: Ricca Chemical

Suitable for use in the following compendial methods: -APHA 4500-ClO2 C, Reagent 3 -APHA 4500-Cl D, Reagent 3b -APHA 4500-ClO2 E, Reagent 3c

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