Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade
Supplier: VWR
Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.
Expand 3 Items
VWR® TRIS-Borate-EDTA Buffer, 5X Concentrate, Ultra Pure Grade
Supplier: VWR
TBE is optimized for DNA electrophoresis techniques using both acrylamide and agarose
Expand 1 Items
VWR® Rapid Transfer Buffer, 10X
Supplier: VWR
Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications
Expand 1 Items
VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade
Supplier: VWR
1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.
Expand 2 Items
VWR® TRIS Buffer, 0.1 M Solution, pH 7.4
Supplier: VWR
Cell culture buffer, TRIS Buffer Solution 0.1M, pH 7.4
Expand 1 Items
VWR® MOPS Buffer Solution 10X, Biotechnology Grade
Supplier: VWR
Optimized for RNA Agarose Electrophoresis
Expand 1 Items
VWR® SDS-Page Gel Buffers
Supplier: VWR
The 4X concentrated buffer (0.5M Tris pH 6.8 and 0.4% SDS) for preparation of stacking gels for Laemmli SDS-Page
Expand 1 Items
J.T.Baker® Cell Lysis Solution, Biotech Reagent
Supplier: AVANTOR PERFORMANCE MATERIAL LLC
J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.
Expand 5 Items
J.T.Baker®, pH Buffer Solutions
Supplier: AVANTOR PERFORMANCE MATERIAL LLC
High quality buffer solutions ensure accurate pH measurements time after time.
Expand 8 Items
VWR® TRIS Glycine Buffer, 10X Liquid Concentrate
Supplier: VWR
A 1X working solution contains 0.025M Tris base and 0.192 M Glycine.
Expand 2 Items
Phosphate Buffered Saline
Supplier: Ward's Science
Cell culture buffer, Phosphate Buffered Saline
Expand 2 Items
VWR® Buffer Solutions
Supplier: VWR
These buffer solutions are for calibrating pH meters
Expand 5 Items
VWR® TRIS-Borate-EDTA Buffer, 10X Liquid Concentrate, Ultra Pure Grade
Supplier: VWR
TBE is optimized for DNA electrophoresis techniques using both acrylamide and agarose.A concentrated (10X), pH adjusted solution of Ultra Pure Grade Tris Buffer, ACS Grade Boric Acid & EDTA which when reconstituted to a working (1X) Solution concentration, contains 0.089M Tris, 0.089M Borate & 0.002M EDTA.
Expand 4 Items
VWR® Phosphate Buffered Saline, Biotechnology Grade
Supplier: VWR
1X Solution Composition:137 mM NaCl, 2.7 mM KCl, 12 mM Phosphate buffer
Expand 1 Items
VWR® HEPES, 1M, pH 7.3, Biotechnology Grade
Supplier: VWR
HEPES, 1M, pH 7.3, VWR®
Expand 2 Items
#: CA21905-026
Supplier: Kimberly-Clark