Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

His Buffer Kit, Cytiva

Supplier: Cytiva

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.

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Lipid Dilution Buffers II

Supplier: Sino Biological

The working solution used for diluting lipid substrates.

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Kinase Dilution Buffers I

Supplier: Sino Biological

Solution of buffer used for diluting kinase protein.

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Corning® HEPES buffer solution (1 mol/L)

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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Biofluid & Cell Buffer (Red), Zymo Research

Supplier: Zymo Research

Cell culture buffer

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10X Phosphate Buffered Saline, Abcam

Supplier: Abcam

10X Phosphate Buffered Saline is a buffer designed for ELISA assay development.

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Imidazole, 0.5 M Buffer Solution, pH 7.5, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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ACES, 0.5 M Buffer Solutions, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Triton® X-100 lysis buffer, pH 7.4, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Pierce I-PER™, Insect Cell Protein Extraction Reagent, Thermo Scientific

Supplier: Thermo Scientific

I-PER™ Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculoviral-infected insect cells grown in suspension or monolayer culture.