Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
VWR® Neutral pH Polyacrylamide Gel Electrophoresis Buffers
Supplier: VWR
Tris-Acetate-SDS, MOPS-SDS, and MES-SDS are useful for high resolution of proteins on neutral pH SDS-PAGE gels.
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VWR® TRIS-Acetate-EDTA Buffer, Ultra Pure Grade
Supplier: VWR
TBE is optimized for DNA electrophoresis techniques using both acrylamide and agarose
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VWR® EDTA 0.5M, Biotechnology Grade
Supplier: VWR
Cloning Buffer, EDTA, 0.5M Solution, pH 8.0
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VWR® TBS Buffer, 20X Liquid
Supplier: VWR
A 1X solution of Tris Buffered Saline (TBS) contains 140 mM Sodium Chloride, 3.0 mM Potassium Chloride and 25 mM Tris.
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VWR® Rapid Transfer Buffer, 10X
Supplier: VWR
Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications
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VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade
Supplier: VWR
Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.
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VWR® SDS-Page Gel Buffers
Supplier: VWR
The 4X concentrated buffer (0.5M Tris pH 6.8 and 0.4% SDS) for preparation of stacking gels for Laemmli SDS-Page
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VWR® MOPS Buffer Solution 10X, Biotechnology Grade
Supplier: VWR
Optimized for RNA Agarose Electrophoresis
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VWR® TRIS-Borate-EDTA Buffer, 5X Concentrate, Ultra Pure Grade
Supplier: VWR
TBE is optimized for DNA electrophoresis techniques using both acrylamide and agarose
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VWR® Phosphate Buffered Saline (PBS) 20X, Ultra Pure Grade
Supplier: VWR
1X Solution Composition:135 mM NaCl, 2.7 mM KCl,11 mM Phosphate Buffer.Does not contain magnesium or calcium.
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VWR® TRIS Buffer, 0.1 M Solution, pH 7.4
Supplier: VWR
Cell culture buffer, TRIS Buffer Solution 0.1M, pH 7.4
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J.T.Baker® Cell Lysis Solution, Biotech Reagent
Supplier: AVANTOR PERFORMANCE MATERIAL LLC
J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.
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J.T.Baker®, pH Buffer Solutions
Supplier: AVANTOR PERFORMANCE MATERIAL LLC
High quality buffer solutions ensure accurate pH measurements time after time.
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VWR® Ultra Pure Grade Phosphate Buffered Saline
Supplier: VWR
1X Solution Composition:137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer
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VWR® READY-PACK™ Ultra Pure Grade TRIS Buffered Saline (TBS)
Supplier: VWR
Pre-blended TBS buffer
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Catalog #: CA21905-026
Supplier: Kimberly-Clark