Restriction Enzymes
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
Live Restriction Enzymes
Supplier: Avantor
These restriction enzymes are used to recognize specific sequences in double strand DNA. BamH I recognizes 5-G/GATCC-3, Ecor I recognizes 5-G/AATTC-3, and Hind III recognizes 5-A/AGCTT-3. The activity level of all our restriction enzymes is 10 units per 1 µl.
All are shipped next day on ice. As they are perishable, a ship date must be specified.
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Dpn I, Restriction Enzymes, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Dpn I is an ideal enzyme for cloning and southern blot analysis.
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BsaXI
Supplier: New England Biolabs (NEB)
Bacillus stearothermophilus 25B (Z
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EcoRI, Restriction Enzymes, from E.coli, New England Biolabs
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the cloned EcoRI gene from E. coli RY13 (R.N. Yoshimori).
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NcoI-HF®, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the cloned and modified from Nocardia carolina (ATCC 19070).
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SpeI-HF®, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
An E. coli strain that carries the cloned and modified SpeI gene from Sphaerotilus species (ATCC 13923). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart® Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver™ qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
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Acc65I
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the cloned Acc65I gene from Acinetobacter calcoaceticus 65 (S.K. Degtyarev).
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SalI, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the SalI gene from Streptomyces albus G (ATCC 49789).
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PvuII
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the PvuII gene from Proteus vulgaris (ATCC 13315).
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BsmFI
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the cloned BsmFI gene from Bacillus stearothermophilus F (ER2683).
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BpmI
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the cloned BpmI gene from Bacillus pumilus (S.K. Degtyarev).
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EcoRV-HF®, Restriction Enzymes, New England Biolabs
Supplier: New England Biolabs (NEB)
EcoRV-HF® is produced in E. coli from a strain that carries the cloned and modified EcoRV gene from the plasmid J62 pLG74 (L.I. Glatman).
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NcoI
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the NcoI gene from Nocardia corallina (ATCC 19070).
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MboI
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the MboI gene from Moraxella bovis (ATCC 10900).
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HaeIII, New England Biolabs
Supplier: New England Biolabs (NEB)
An E. coli strain that carries the HaeIII gene from Haemophilus aegypticus (ATCC 11116).
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DdeI, New England Biolabs
Supplier: New England Biolabs (NEB)
An E.coli strain that carries the DdeI gene from Desulfovibrio desulfuricans (NCIB 83120).
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