You searched for: SMCC (N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate)

Supplier: TCI America

CAS Number: 64987-85-5
MDL Number: MFCD00009634
Molecular Formula: C16H18N2O6
Molecular Weight: 334.33
Purity/Analysis Method: >98.0% (HPLC)
Form: Crystal
Color: White
Melting point (°C): 177
Storage Temperature: <0°C

Supplier: MilliporeSigma

Heterobifunctional reagent useful for enzyme labeling of antibodies and antibody fragments. Cross-links both a sulfhydryl group and a free amine group. Useful for sequential conjugation to minimize polymerization.
Reacts with primary amines and sulfhydryl groups.

Supplier: G-Biosciences

Succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate Heterobifunctional Cross Linkers.

Supplier: Thermo Scientific

Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.