Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
Monarch® Precipitation Enhancer, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® precipitation enhancer solution, supplied as a component of the Monarch® HMW DNA extraction kit for cells and blood, is a salt-based solution that facilitates the precipitation of DNA onto the Monarch® DNA capture beads during HMW DNA extraction from cells and blood.
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Luna Cell Ready Lysis Module, New England Biolabs
Supplier: New England Biolabs (NEB)
Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. The Luna Cell Ready Lysis Module is a fast, convenient, and sensitive alternative for evaluating RNA expression levels without performing traditional RNA extraction steps.
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Phosphate buffered RIPA (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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ToxiLight® 100% Lysis Control Set
Supplier: LONZA PHARMA - BIOSCIENCE CA
The ToxiLight® 100% lysis reagent set is intended for use with the ToxiLight® non-destructive cytotoxicity assay (sold seperately) and is designed to give the user a 100% lysis or total adenylate kinase control.
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Sodium Dodecyl Sulfate SDS, 20%
Supplier: Rockland Immunochemical
SDS, sodium dodecyl sulfate is an anionic surfactant.
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FOCUS™ Extraction Buffers, G-Biosciences
Supplier: G-Biosciences
One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers
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Monarch® Buffer B1
Supplier: New England Biolabs (NEB)
Monarch Buffer B1 is included in Monarch nucleic acid purification kits, and also offered as a standalone buffer for convenience.
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Gram Negative Lysis Buffer, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Gram-negative Lysis Buffer is an extraction buffer for soluble proteins from Gram-negative bacteria
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Monarch® RNase A, New England Biolabs
Supplier: New England Biolabs (NEB)
Monarch® RNase A is a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify genomic DNA from a variety of sample types.
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Monarch® gDNA Nuclei Prep and Lysis Buffer Pack, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® gDNA Nuclei Prep and lysis buffer pack contains two lysis buffers used in the Monarch HMW DNA extraction kit for cells and blood.
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RIPA Lysis Buffer, Rockland Immunochemicals
Supplier: Rockland Immunochemical
RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
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Cell Lysis Buffer, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Cell Lysis Buffer is used to lyse cells under non-denaturing conditions.
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RIPA Lysis and Extraction Buffer, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' RIPA Lysis and Extraction Buffer is a complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells
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Monarch® gDNA Elution Buffer II, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® gDNA elution buffer II is supplied as a component of the Monarch HMW DNA extraction kits.
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Monarch® gDNA Wash Buffer, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® gDNA Wash Buffer a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify up to 30 µg genomic DNA from a variety of sample types.
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Monarch® HMW gDNA Tissue Lysis Buffer, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® HMW gDNA tissue lysis buffer, supplied as a component of the Monarch HMW DNA extraction kit for tissue, is a highly effective lysis buffer for tissue and bacteria samples. Its composition ensures immediate and complete inactivation of nucleases in the samples at the start of the lysis.
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Catalog #: CA21905-026
Supplier: Kimberly-Clark